Genetic Dissection of Heritable Traits in Yeast Using Bulk Segregant Analysis.

Published

Journal Article

Bulk segregant analysis (BSA) is commonly used to determine the genetic basis of complex traits in yeast. This technique involves phenotyping progeny from a cross and then selectively genotyping pooled subsets of offspring with extreme phenotypes. Analysis of these genotype data can identify loci that show skewed allele frequencies in a group of phenotypically extreme individuals and that are likely to contribute to a trait. BSA can be applied to diverse strain crosses, including ones involving nonreference isolates. Further, given the high throughput of next-generation sequencing, it is possible to conduct many BSA experiments in parallel. Here, we present a BSA protocol for the generation of recombinant cross progeny. We then describe general BSA strategies for conducting phenotyping, causal loci detection, and candidate gene identification in a statistically powerful manner.

Full Text

Duke Authors

Cited Authors

  • Ehrenreich, IM; Magwene, PM

Published Date

  • June 2017

Published In

Volume / Issue

  • 2017 / 6

Start / End Page

  • pdb.prot088989 -

PubMed ID

  • 28572183

Pubmed Central ID

  • 28572183

Electronic International Standard Serial Number (EISSN)

  • 1559-6095

International Standard Serial Number (ISSN)

  • 1940-3402

Digital Object Identifier (DOI)

  • 10.1101/pdb.prot088989

Language

  • eng