The effect of timing of influenza vaccination and sample collection on antibody titers and responses in the aged.

Journal Article (Journal Article)

Antibody responses, B cell subset distribution in blood and the blood transcriptome were analyzed in younger and aged human subjects before and after vaccination with the inactivated influenza vaccine. In the aged, but not the younger, individuals we saw a clear difference in antibody titers including those at baseline depending on the time of vaccination and sample collection. Differences in baseline titers in aged individuals treated in the morning or afternoon in turn affected responsiveness to the vaccine. In both younger and aged individuals, the time of sample collection also affected relative numbers of some of the B cell subsets in blood. A global gene expression analysis with whole blood samples from the aged showed small but statistically significant differences depending on the time of sample collection. Our data do not indicate that timing of vaccination affects immune responsiveness of the aged, but rather shows that in clinical influenza vaccine trials timing of collection of samples can have a major and potentially misleading influence on study outcome. In future vaccine trials, timing of vaccination and sample collection should be recorded carefully to allow for its use as a study covariant.

Full Text

Duke Authors

Cited Authors

  • Kurupati, RK; Kossenkoff, A; Kannan, S; Haut, LH; Doyle, S; Yin, X; Schmader, KE; Liu, Q; Showe, L; Ertl, HCJ

Published Date

  • June 27, 2017

Published In

Volume / Issue

  • 35 / 30

Start / End Page

  • 3700 - 3708

PubMed ID

  • 28583307

Electronic International Standard Serial Number (EISSN)

  • 1873-2518

Digital Object Identifier (DOI)

  • 10.1016/j.vaccine.2017.05.074


  • eng

Conference Location

  • Netherlands