Robust parametric and semi-parametric spot fitting for spot array images.
Publication
, Journal Article
Brändle, N; Chen, HY; Bischof, H; Lapp, H
Published in: Proceedings. International Conference on Intelligent Systems for Molecular Biology
January 2000
In this paper we address the problem of reliably fitting parametric and semi-parametric models to spots in high density spot array images obtained in gene expression experiments. The goal is to measure the amount of label bound to an array element. A lot of spots can be modelled accurately by a Gaussian shape. In order to deal with highly overlapping spots we use robust M-estimators. When the parametric method fails (which can be detected automatically) we use a novel, robust semi-parametric method which can handle spots of different shapes accurately. The introduced techniques are evaluated experimentally.
Duke Scholars
Published In
Proceedings. International Conference on Intelligent Systems for Molecular Biology
ISSN
1553-0833
Publication Date
January 2000
Volume
8
Start / End Page
46 / 56
Related Subject Headings
- Oligonucleotide Array Sequence Analysis
- Image Processing, Computer-Assisted
- Humans
- Gene Expression Profiling
- Animals
Citation
APA
Chicago
ICMJE
MLA
NLM
Brändle, N., Chen, H. Y., Bischof, H., & Lapp, H. (2000). Robust parametric and semi-parametric spot fitting for spot array images. Proceedings. International Conference on Intelligent Systems for Molecular Biology, 8, 46–56.
Brändle, N., H. Y. Chen, H. Bischof, and H. Lapp. “Robust parametric and semi-parametric spot fitting for spot array images.” Proceedings. International Conference on Intelligent Systems for Molecular Biology 8 (January 2000): 46–56.
Brändle N, Chen HY, Bischof H, Lapp H. Robust parametric and semi-parametric spot fitting for spot array images. Proceedings International Conference on Intelligent Systems for Molecular Biology. 2000 Jan;8:46–56.
Brändle, N., et al. “Robust parametric and semi-parametric spot fitting for spot array images.” Proceedings. International Conference on Intelligent Systems for Molecular Biology, vol. 8, Jan. 2000, pp. 46–56.
Brändle N, Chen HY, Bischof H, Lapp H. Robust parametric and semi-parametric spot fitting for spot array images. Proceedings International Conference on Intelligent Systems for Molecular Biology. 2000 Jan;8:46–56.
Published In
Proceedings. International Conference on Intelligent Systems for Molecular Biology
ISSN
1553-0833
Publication Date
January 2000
Volume
8
Start / End Page
46 / 56
Related Subject Headings
- Oligonucleotide Array Sequence Analysis
- Image Processing, Computer-Assisted
- Humans
- Gene Expression Profiling
- Animals