The Top1 paradox: Friend and foe of the eukaryotic genome.

Journal Article (Journal Article;Review)

Topoisomerases manage the torsional stress associated with the separation of DNA strands during transcription and DNA replication. Eukaryotic Topoisomerase I (Top1) is a Type IB enzyme that nicks and rejoins only one strand of duplex DNA, and it is especially important during transcription. By resolving transcription-associated torsional stress, Top1 reduces the accumulation of genome-destabilizing R-loops and non-B DNA structures. The DNA nicking activity of Top1, however, can also initiate genome instability in the form of illegitimate recombination, homologous recombination and mutagenesis. In this review, we focus on the diverse, and often opposing, roles of Top1 in regulating eukaryotic genome stability.

Full Text

Duke Authors

Cited Authors

  • Kim, N; Jinks-Robertson, S

Published Date

  • August 2017

Published In

Volume / Issue

  • 56 /

Start / End Page

  • 33 - 41

PubMed ID

  • 28641942

Pubmed Central ID

  • PMC5560766

Electronic International Standard Serial Number (EISSN)

  • 1568-7856

Digital Object Identifier (DOI)

  • 10.1016/j.dnarep.2017.06.005


  • eng

Conference Location

  • Netherlands