Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma.
Journal Article (Journal Article)
Genetically engineered mouse models that employ site-specific recombinase technology are important tools for cancer research but can be costly and time-consuming. The CRISPR-Cas9 system has been adapted to generate autochthonous tumours in mice, but how these tumours compare to tumours generated by conventional recombinase technology remains to be fully explored. Here we use CRISPR-Cas9 to generate multiple subtypes of primary sarcomas efficiently in wild type and genetically engineered mice. These data demonstrate that CRISPR-Cas9 can be used to generate multiple subtypes of soft tissue sarcomas in mice. Primary sarcomas generated with CRISPR-Cas9 and Cre recombinase technology had similar histology, growth kinetics, copy number variation and mutational load as assessed by whole exome sequencing. These results show that sarcomas generated with CRISPR-Cas9 technology are similar to sarcomas generated with conventional modelling techniques and suggest that CRISPR-Cas9 can be used to more rapidly generate genotypically and phenotypically similar cancers.
Full Text
Duke Authors
- Cardona, Diana Marcella
- Dave, Sandeep S.
- Eward, William Curtis
- Gersbach, Charles
- Kirsch, David Guy
- Mowery, Yvonne Marie
- Van Mater, David Sinclaire
Cited Authors
- Huang, J; Chen, M; Whitley, MJ; Kuo, H-C; Xu, ES; Walens, A; Mowery, YM; Van Mater, D; Eward, WC; Cardona, DM; Luo, L; Ma, Y; Lopez, OM; Nelson, CE; Robinson-Hamm, JN; Reddy, A; Dave, SS; Gersbach, CA; Dodd, RD; Kirsch, DG
Published Date
- July 10, 2017
Published In
Volume / Issue
- 8 /
Start / End Page
- 15999 -
PubMed ID
- 28691711
Pubmed Central ID
- PMC5508130
Electronic International Standard Serial Number (EISSN)
- 2041-1723
Digital Object Identifier (DOI)
- 10.1038/ncomms15999
Language
- eng
Conference Location
- England