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Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma.

Publication ,  Journal Article
Huang, J; Chen, M; Whitley, MJ; Kuo, H-C; Xu, ES; Walens, A; Mowery, YM; Van Mater, D; Eward, WC; Cardona, DM; Luo, L; Ma, Y; Lopez, OM ...
Published in: Nat Commun
July 10, 2017

Genetically engineered mouse models that employ site-specific recombinase technology are important tools for cancer research but can be costly and time-consuming. The CRISPR-Cas9 system has been adapted to generate autochthonous tumours in mice, but how these tumours compare to tumours generated by conventional recombinase technology remains to be fully explored. Here we use CRISPR-Cas9 to generate multiple subtypes of primary sarcomas efficiently in wild type and genetically engineered mice. These data demonstrate that CRISPR-Cas9 can be used to generate multiple subtypes of soft tissue sarcomas in mice. Primary sarcomas generated with CRISPR-Cas9 and Cre recombinase technology had similar histology, growth kinetics, copy number variation and mutational load as assessed by whole exome sequencing. These results show that sarcomas generated with CRISPR-Cas9 technology are similar to sarcomas generated with conventional modelling techniques and suggest that CRISPR-Cas9 can be used to more rapidly generate genotypically and phenotypically similar cancers.

Duke Scholars

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Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

July 10, 2017

Volume

8

Start / End Page

15999

Location

England

Related Subject Headings

  • Sarcoma, Experimental
  • Neurilemmoma
  • NIH 3T3 Cells
  • Mutation
  • Mice, Nude
  • Mice
  • Male
  • Integrases
  • Gene Editing
  • Electroporation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Huang, J., Chen, M., Whitley, M. J., Kuo, H.-C., Xu, E. S., Walens, A., … Kirsch, D. G. (2017). Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma. Nat Commun, 8, 15999. https://doi.org/10.1038/ncomms15999
Huang, Jianguo, Mark Chen, Melodi Javid Whitley, Hsuan-Cheng Kuo, Eric S. Xu, Andrea Walens, Yvonne M. Mowery, et al. “Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma.Nat Commun 8 (July 10, 2017): 15999. https://doi.org/10.1038/ncomms15999.
Huang J, Chen M, Whitley MJ, Kuo H-C, Xu ES, Walens A, et al. Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma. Nat Commun. 2017 Jul 10;8:15999.
Huang, Jianguo, et al. “Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma.Nat Commun, vol. 8, July 2017, p. 15999. Pubmed, doi:10.1038/ncomms15999.
Huang J, Chen M, Whitley MJ, Kuo H-C, Xu ES, Walens A, Mowery YM, Van Mater D, Eward WC, Cardona DM, Luo L, Ma Y, Lopez OM, Nelson CE, Robinson-Hamm JN, Reddy A, Dave SS, Gersbach CA, Dodd RD, Kirsch DG. Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma. Nat Commun. 2017 Jul 10;8:15999.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

July 10, 2017

Volume

8

Start / End Page

15999

Location

England

Related Subject Headings

  • Sarcoma, Experimental
  • Neurilemmoma
  • NIH 3T3 Cells
  • Mutation
  • Mice, Nude
  • Mice
  • Male
  • Integrases
  • Gene Editing
  • Electroporation