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Epigenome-wide association of PTSD from heterogeneous cohorts with a common multi-site analysis pipeline.

Publication ,  Journal Article
Ratanatharathorn, A; Boks, MP; Maihofer, AX; Aiello, AE; Amstadter, AB; Ashley-Koch, AE; Baker, DG; Beckham, JC; Bromet, E; Dennis, M; Geuze, E ...
Published in: Am J Med Genet B Neuropsychiatr Genet
September 2017

Compelling evidence suggests that epigenetic mechanisms such as DNA methylation play a role in stress regulation and in the etiologic basis of stress related disorders such as Post traumatic Stress Disorder (PTSD). Here we describe the purpose and methods of an international consortium that was developed to study the role of epigenetics in PTSD. Inspired by the approach used in the Psychiatric Genomics Consortium, we brought together investigators representing seven cohorts with a collective sample size of N = 1147 that included detailed information on trauma exposure, PTSD symptoms, and genome-wide DNA methylation data. The objective of this consortium is to increase the analytical sample size by pooling data and combining expertise so that DNA methylation patterns associated with PTSD can be identified. Several quality control and analytical pipelines were evaluated for their control of genomic inflation and technical artifacts with a joint analysis procedure established to derive comparable data over the cohorts for meta-analysis. We propose methods to deal with ancestry population stratification and type I error inflation and discuss the advantages and disadvantages of applying robust error estimates. To evaluate our pipeline, we report results from an epigenome-wide association study (EWAS) of age, which is a well-characterized phenotype with known epigenetic associations. Overall, while EWAS are highly complex and subject to similar challenges as genome-wide association studies (GWAS), we demonstrate that an epigenetic meta-analysis with a relatively modest sample size can be well-powered to identify epigenetic associations. Our pipeline can be used as a framework for consortium efforts for EWAS.

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Published In

Am J Med Genet B Neuropsychiatr Genet

DOI

EISSN

1552-485X

Publication Date

September 2017

Volume

174

Issue

6

Start / End Page

619 / 630

Location

United States

Related Subject Headings

  • Stress Disorders, Post-Traumatic
  • Phenotype
  • Middle Aged
  • Male
  • Humans
  • Genomics
  • Genome-Wide Association Study
  • Genetic Predisposition to Disease
  • Female
  • Epigenomics
 

Citation

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ICMJE
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Ratanatharathorn, A., Boks, M. P., Maihofer, A. X., Aiello, A. E., Amstadter, A. B., Ashley-Koch, A. E., … Smith, A. K. (2017). Epigenome-wide association of PTSD from heterogeneous cohorts with a common multi-site analysis pipeline. Am J Med Genet B Neuropsychiatr Genet, 174(6), 619–630. https://doi.org/10.1002/ajmg.b.32568
Ratanatharathorn, Andrew, Marco P. Boks, Adam X. Maihofer, Allison E. Aiello, Ananda B. Amstadter, Allison E. Ashley-Koch, Dewleen G. Baker, et al. “Epigenome-wide association of PTSD from heterogeneous cohorts with a common multi-site analysis pipeline.Am J Med Genet B Neuropsychiatr Genet 174, no. 6 (September 2017): 619–30. https://doi.org/10.1002/ajmg.b.32568.
Ratanatharathorn A, Boks MP, Maihofer AX, Aiello AE, Amstadter AB, Ashley-Koch AE, et al. Epigenome-wide association of PTSD from heterogeneous cohorts with a common multi-site analysis pipeline. Am J Med Genet B Neuropsychiatr Genet. 2017 Sep;174(6):619–30.
Ratanatharathorn, Andrew, et al. “Epigenome-wide association of PTSD from heterogeneous cohorts with a common multi-site analysis pipeline.Am J Med Genet B Neuropsychiatr Genet, vol. 174, no. 6, Sept. 2017, pp. 619–30. Pubmed, doi:10.1002/ajmg.b.32568.
Ratanatharathorn A, Boks MP, Maihofer AX, Aiello AE, Amstadter AB, Ashley-Koch AE, Baker DG, Beckham JC, Bromet E, Dennis M, Garrett ME, Geuze E, Guffanti G, Hauser MA, Kilaru V, Kimbrel NA, Koenen KC, Kuan P-F, Logue MW, Luft BJ, Miller MW, Mitchell C, Nugent NR, Ressler KJ, Rutten BPF, Stein MB, Vermetten E, Vinkers CH, Youssef NA, VA Mid-Atlantic MIRECC Workgroup, PGC PTSD Epigenetics Workgroup, Uddin M, Nievergelt CM, Smith AK. Epigenome-wide association of PTSD from heterogeneous cohorts with a common multi-site analysis pipeline. Am J Med Genet B Neuropsychiatr Genet. 2017 Sep;174(6):619–630.
Journal cover image

Published In

Am J Med Genet B Neuropsychiatr Genet

DOI

EISSN

1552-485X

Publication Date

September 2017

Volume

174

Issue

6

Start / End Page

619 / 630

Location

United States

Related Subject Headings

  • Stress Disorders, Post-Traumatic
  • Phenotype
  • Middle Aged
  • Male
  • Humans
  • Genomics
  • Genome-Wide Association Study
  • Genetic Predisposition to Disease
  • Female
  • Epigenomics