Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST).


Journal Article

CEST-NMR spectroscopy is a powerful tool for probing the conformational dynamics of macromolecules. We present a HNdec-CEST experiment that simplifies the relaxation matrix, reduces fitting parameters, and enhances signal resolution. Importantly, fitting of HNdec-CEST profiles enables robust extraction of exchange rates as well as excited-state chemical shifts and populations.

Full Text

Duke Authors

Cited Authors

  • Wu, Q; Fenton, BA; Wojtaszek, JL; Zhou, P

Published Date

  • July 2017

Published In

Volume / Issue

  • 53 / 61

Start / End Page

  • 8541 - 8544

PubMed ID

  • 28707688

Pubmed Central ID

  • 28707688

Electronic International Standard Serial Number (EISSN)

  • 1364-548X

International Standard Serial Number (ISSN)

  • 1359-7345

Digital Object Identifier (DOI)

  • 10.1039/c7cc05021f


  • eng