Attachment of bovine parvovirus to O-linked alpha 2,3 neuraminic acid on glycophorin A.


Journal Article

The bovine parvovirus (BPV) hemagglutinates human erythrocytes by binding to glycophorin A (GPA). The purpose of this study was to determine which carbohydrate on GPA binds BPV. Treatment of GPA with alpha2,3,-6,-8 neuraminidase eliminated binding of BPV to GPA. Beta-elimination of O-linked sialic acids on GPA eliminated binding, while removal of N-linked carbohydrates using the N-glycosidase PNGase F failed to eliminate binding. Treatment of GPA with a neuraminidase which specifically cleaved alpha2,3 glycosidic bonds eliminated BPV binding and, following this treatment, virus binding to GPA was restored by reconstitution of alpha2,3-linked neuraminic acids. These results indicated the O-linked alpha2,3 neuraminic acids of GPA bind BPV.

Full Text

Duke Authors

Cited Authors

  • Blackburn, SD; Cline, SE; Hemming, JP; Johnson, FB

Published Date

  • July 2005

Published In

Volume / Issue

  • 150 / 7

Start / End Page

  • 1477 - 1484

PubMed ID

  • 15750863

Pubmed Central ID

  • 15750863

International Standard Serial Number (ISSN)

  • 0304-8608

Digital Object Identifier (DOI)

  • 10.1007/s00705-005-0496-y


  • eng

Conference Location

  • Austria