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Nanosensors for nucleic acid targets detection using SERS

Publication ,  Conference
Strobbia, P; Crawford, BM; Wang, HN; Vo-Dinh, T
Published in: Proceedings of SPIE - The International Society for Optical Engineering
January 1, 2017

Single cell analysis can aid the study of molecular events responsible for cellular functions and unveil their connections to the biological functions of an organism. Biosensors based on surface enhanced Raman spectroscopy (SERS) can be used to this end and offer several advantages over other sensing platforms, such as sensitivity and multiplexed capabilities, among others. While SERS nanosensors/nanoparticles have been used for analysis in single cells, the delivery of such biosensors relies on cellular uptake, which requires long incubation time and has different efficiencies among cell lines. Nanosensors based on tapered optical fibers, instead, can be inserted in single cells and detect target molecules in infcellular compartment. The combination of these sensing devices with the transduction mechanism of nucleic acid based nanoprobes (i.e. inverse molecular sentinels) will permit the more direct detection of nucleic acids within single cells. This paper presents the development of tapered fiber-based biosensors for the detection of nucleic acid biomarkers in plant cells. The use of inverse molecular sentinels in plant cell was demonstrated. Sensors based on tapered fibers were fabricated and used to measure SERS from a single cell.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

EISSN

1996-756X

ISSN

0277-786X

ISBN

9781510609310

Publication Date

January 1, 2017

Volume

10215

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

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Strobbia, P., Crawford, B. M., Wang, H. N., & Vo-Dinh, T. (2017). Nanosensors for nucleic acid targets detection using SERS. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 10215). https://doi.org/10.1117/12.2267757
Strobbia, P., B. M. Crawford, H. N. Wang, and T. Vo-Dinh. “Nanosensors for nucleic acid targets detection using SERS.” In Proceedings of SPIE - The International Society for Optical Engineering, Vol. 10215, 2017. https://doi.org/10.1117/12.2267757.
Strobbia P, Crawford BM, Wang HN, Vo-Dinh T. Nanosensors for nucleic acid targets detection using SERS. In: Proceedings of SPIE - The International Society for Optical Engineering. 2017.
Strobbia, P., et al. “Nanosensors for nucleic acid targets detection using SERS.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 10215, 2017. Scopus, doi:10.1117/12.2267757.
Strobbia P, Crawford BM, Wang HN, Vo-Dinh T. Nanosensors for nucleic acid targets detection using SERS. Proceedings of SPIE - The International Society for Optical Engineering. 2017.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

EISSN

1996-756X

ISSN

0277-786X

ISBN

9781510609310

Publication Date

January 1, 2017

Volume

10215

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering