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Aerosol transfer of bladder urothelial and smooth muscle cells onto demucosalized colonic segments for bladder augmentation: in vivo, long term, and functional pilot study.

Publication ,  Journal Article
Hidas, G; Lee, HJ; Bahoric, A; Kelly, MS; Watts, B; Liu, Z; Saharti, S; Lusch, A; Alamsahebpour, A; Kerbl, D; Truong, H; Zi, X; Khoury, AE
Published in: Journal of pediatric urology
October 2015

Bladder augmentation technique has changed over the years and the current practice has significant adverse health effects and long-term sequelae. Previously, we reported a novel cell transfer technology for covering demucosalized colonic segments with bladder urothelium and smooth muscle cells through an aerosol spraying of these cells and a fibrin glue mixture.To determine the long-term durability and functional characteristics of demucosalized segments of colon repopulated with urothelial cells in the bladder of swine for use in augmentation cystoplasty.Nine swine were divided into three groups. The first group (control) underwent standard colocystoplasty; the second group underwent colocystoplasty with colonic demucosalization and aerosol application of fibrin glue and urothelial cell mixture; in the third group detrusor cells were added to the mixture described in group two. The animals were kept for 6 months. Absorptive and secretory function was assessed. Bladders were harvested for histological and immunohistochemical evaluation.All animals but one in the experimental groups showed confluent urothelial coverage of the colonic segment in the bladder without any evidence of fibrosis, inflammation, or regrowth of colonic epithelial cells. Ten percent of the instilled water in the bladder was absorbed within an hour in the control group, but none in experimental groups(p = 0.02). The total urine sediment and protein contents were higher in the control group compared with experimental groups (p < 0.05).Both study groups developed a uniform urothelial lining. Histologically, the group with smooth muscle had an added layer of submucosal smooth muscle. Six months after bladder augmentation the new lining was durable. We were also able to demonstrate that the reconstituted augmented segments secrete and absorb significantly less than the control colocystoplasty group. We used a non-validated simple method to evaluate permeability of the new urothelial lining to water. To determine if the aerosol transfer of bladder cells would have behaved differently in the neurogenic bladder population, this experiment should have been performed in animals with neuropathic bladders.Aerosol spraying of single cell suspension of urothelial and muscular cells with fibrin glue resulted in coverage of the demucosalized intestinal segment with a uniform urothelial layer. This new lining segment was durable without regrowth of colonic mucosa after 6 months. The new reconstituted segment absorbs and secretes significantly less than control colocystoplasty.

Duke Scholars

Published In

Journal of pediatric urology

DOI

EISSN

1873-4898

ISSN

1477-5131

Publication Date

October 2015

Volume

11

Issue

5

Start / End Page

260.e1 / 260.e6

Related Subject Headings

  • Urothelium
  • Urology & Nephrology
  • Urologic Surgical Procedures
  • Urinary Bladder, Neurogenic
  • Urinary Bladder
  • Transplantation, Autologous
  • Time Factors
  • Swine
  • Pilot Projects
  • Muscle, Smooth
 

Citation

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Hidas, G., Lee, H. J., Bahoric, A., Kelly, M. S., Watts, B., Liu, Z., … Khoury, A. E. (2015). Aerosol transfer of bladder urothelial and smooth muscle cells onto demucosalized colonic segments for bladder augmentation: in vivo, long term, and functional pilot study. Journal of Pediatric Urology, 11(5), 260.e1-260.e6. https://doi.org/10.1016/j.jpurol.2015.02.020
Hidas, Guy, Hak J. Lee, Andrej Bahoric, Maryellen S. Kelly, Blake Watts, Zhongbo Liu, Samah Saharti, et al. “Aerosol transfer of bladder urothelial and smooth muscle cells onto demucosalized colonic segments for bladder augmentation: in vivo, long term, and functional pilot study.Journal of Pediatric Urology 11, no. 5 (October 2015): 260.e1-260.e6. https://doi.org/10.1016/j.jpurol.2015.02.020.
Hidas G, Lee HJ, Bahoric A, Kelly MS, Watts B, Liu Z, et al. Aerosol transfer of bladder urothelial and smooth muscle cells onto demucosalized colonic segments for bladder augmentation: in vivo, long term, and functional pilot study. Journal of pediatric urology. 2015 Oct;11(5):260.e1-260.e6.
Hidas, Guy, et al. “Aerosol transfer of bladder urothelial and smooth muscle cells onto demucosalized colonic segments for bladder augmentation: in vivo, long term, and functional pilot study.Journal of Pediatric Urology, vol. 11, no. 5, Oct. 2015, pp. 260.e1-260.e6. Epmc, doi:10.1016/j.jpurol.2015.02.020.
Hidas G, Lee HJ, Bahoric A, Kelly MS, Watts B, Liu Z, Saharti S, Lusch A, Alamsahebpour A, Kerbl D, Truong H, Zi X, Khoury AE. Aerosol transfer of bladder urothelial and smooth muscle cells onto demucosalized colonic segments for bladder augmentation: in vivo, long term, and functional pilot study. Journal of pediatric urology. 2015 Oct;11(5):260.e1-260.e6.
Journal cover image

Published In

Journal of pediatric urology

DOI

EISSN

1873-4898

ISSN

1477-5131

Publication Date

October 2015

Volume

11

Issue

5

Start / End Page

260.e1 / 260.e6

Related Subject Headings

  • Urothelium
  • Urology & Nephrology
  • Urologic Surgical Procedures
  • Urinary Bladder, Neurogenic
  • Urinary Bladder
  • Transplantation, Autologous
  • Time Factors
  • Swine
  • Pilot Projects
  • Muscle, Smooth