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Relative contribution of PECAM-1 adhesion and signaling to the maintenance of vascular integrity.

Publication ,  Journal Article
Privratsky, JR; Paddock, CM; Florey, O; Newman, DK; Muller, WA; Newman, PJ
Published in: J Cell Sci
May 1, 2011

PECAM-1 (CD31) is a cellular adhesion and signaling receptor that is highly expressed at endothelial cell-cell junctions in confluent vascular beds. Previous studies have implicated PECAM-1 in the maintenance of vascular barrier integrity; however, the mechanisms behind PECAM-1-mediated barrier protection are still poorly understood. The goal of the present study, therefore, was to examine the pertinent biological properties of PECAM-1 (i.e. adhesion and/or signaling) that allow it to support barrier integrity. We found that, compared with PECAM-1-deficient endothelial cells, PECAM-1-expressing endothelial cell monolayers exhibit increased steady-state barrier function, as well as more rapid restoration of barrier integrity following thrombin-induced perturbation of the endothelial cell monolayer. The majority of PECAM-1-mediated barrier protection was found to be due to the ability of PECAM-1 to interact homophilically and become localized to cell-cell junctions, because a homophilic binding-crippled mutant form of PECAM-1 was unable to support efficient barrier function when re-expressed in cells. By contrast, cells expressing PECAM-1 variants lacking residues known to be involved in PECAM-1-mediated signal transduction exhibited normal to near-normal barrier integrity. Taken together, these studies suggest that PECAM-1-PECAM-1 homophilic interactions are more important than its signaling function for maintaining the integrity of endothelial cell junctions.

Duke Scholars

Published In

J Cell Sci

DOI

EISSN

1477-9137

Publication Date

May 1, 2011

Volume

124

Issue

Pt 9

Start / End Page

1477 / 1485

Location

England

Related Subject Headings

  • Signal Transduction
  • Protein Binding
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Intercellular Junctions
  • Humans
  • Endothelial Cells
  • Developmental Biology
  • Cell Line
  • Cell Adhesion
  • 3101 Biochemistry and cell biology
 

Citation

APA
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ICMJE
MLA
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Privratsky, J. R., Paddock, C. M., Florey, O., Newman, D. K., Muller, W. A., & Newman, P. J. (2011). Relative contribution of PECAM-1 adhesion and signaling to the maintenance of vascular integrity. J Cell Sci, 124(Pt 9), 1477–1485. https://doi.org/10.1242/jcs.082271
Privratsky, Jamie R., Cathy M. Paddock, Oliver Florey, Debra K. Newman, William A. Muller, and Peter J. Newman. “Relative contribution of PECAM-1 adhesion and signaling to the maintenance of vascular integrity.J Cell Sci 124, no. Pt 9 (May 1, 2011): 1477–85. https://doi.org/10.1242/jcs.082271.
Privratsky JR, Paddock CM, Florey O, Newman DK, Muller WA, Newman PJ. Relative contribution of PECAM-1 adhesion and signaling to the maintenance of vascular integrity. J Cell Sci. 2011 May 1;124(Pt 9):1477–85.
Privratsky, Jamie R., et al. “Relative contribution of PECAM-1 adhesion and signaling to the maintenance of vascular integrity.J Cell Sci, vol. 124, no. Pt 9, May 2011, pp. 1477–85. Pubmed, doi:10.1242/jcs.082271.
Privratsky JR, Paddock CM, Florey O, Newman DK, Muller WA, Newman PJ. Relative contribution of PECAM-1 adhesion and signaling to the maintenance of vascular integrity. J Cell Sci. 2011 May 1;124(Pt 9):1477–1485.
Journal cover image

Published In

J Cell Sci

DOI

EISSN

1477-9137

Publication Date

May 1, 2011

Volume

124

Issue

Pt 9

Start / End Page

1477 / 1485

Location

England

Related Subject Headings

  • Signal Transduction
  • Protein Binding
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Intercellular Junctions
  • Humans
  • Endothelial Cells
  • Developmental Biology
  • Cell Line
  • Cell Adhesion
  • 3101 Biochemistry and cell biology