Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood.

Journal Article (Journal Article)

The ELISA is the mainstay for sensitive and quantitative detection of protein analytes. Despite its utility, ELISA is time-consuming, resource-intensive, and infrastructure-dependent, limiting its availability in resource-limited regions. Here, we describe a self-contained immunoassay platform (the "D4 assay") that converts the sandwich immunoassay into a point-of-care test (POCT). The D4 assay is fabricated by inkjet printing assay reagents as microarrays on nanoscale polymer brushes on glass chips, so that all reagents are "on-chip," and these chips show durable storage stability without cold storage. The D4 assay can interrogate multiple analytes from a drop of blood, is compatible with a smartphone detector, and displays analytical figures of merit that are comparable to standard laboratory-based ELISA in whole blood. These attributes of the D4 POCT have the potential to democratize access to high-performance immunoassays in resource-limited settings without sacrificing their performance.

Full Text

Duke Authors

Cited Authors

  • Joh, DY; Hucknall, AM; Wei, Q; Mason, KA; Lund, ML; Fontes, CM; Hill, RT; Blair, R; Zimmers, Z; Achar, RK; Tseng, D; Gordan, R; Freemark, M; Ozcan, A; Chilkoti, A

Published Date

  • August 22, 2017

Published In

Volume / Issue

  • 114 / 34

Start / End Page

  • E7054 - E7062

PubMed ID

  • 28784765

Pubmed Central ID

  • PMC5576789

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.1703200114


  • eng

Conference Location

  • United States