Successful treatment of CCL4-induced acute liver failure with portal vein arterialization in the rat.


Journal Article

INTRODUCTION: Optimization of the conditions for regeneration of the native diseased liver is a major goal in patients with acute liver failure. This study sought to determine whether portal vein arterialization (PVA), which increases the oxygen supply to the liver, was protective in a rat model of liver failure. METHODS: At 24 hours after CCl(4) intoxication, Sprague-Dawley rats (six per group) were assigned to receive PVA or as controls. We determined blood tests, histology, and 10-day survivals. Hepatocyte regeneration was assessed by the mitotic index and bromodeoxyuridine (BrdU) incorporation. RESULTS: Serum transaminases were significantly lower in PVA-treated rats than in control animals: liver necrosis resolved rapidly after PVA. The BrdU staining and mitotic index were severalfold higher among PVA-treated than in untreated rats. Survival was 100% among rats with PVA and 40% in untreated animals (P < .01). CONCLUSIONS: PVA led to resolution of CCl(4)-induced massive liver necrosis in the rat. This effect was probably mediated by activation of rapid and extensive hepatocyte regeneration. PVA might provide a novel, alternative approach to treat acute liver failure.

Full Text

Cited Authors

  • Nardo, B; Puviani, L; Caraceni, P; Pacilè, V; Bertelli, R; Beltempo, P; Cavallari, G; Chieco, P; Pariali, M; Pertosa, AM; Angiolini, G; Domenicali, M; Neri, F; Tsivian, M; Bernardi, M; Cavallari, A

Published Date

  • May 2006

Published In

Volume / Issue

  • 38 / 4

Start / End Page

  • 1187 - 1189

PubMed ID

  • 16757302

Pubmed Central ID

  • 16757302

International Standard Serial Number (ISSN)

  • 0041-1345

Digital Object Identifier (DOI)

  • 10.1016/j.transproceed.2006.03.056


  • eng

Conference Location

  • United States