In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging.

Journal Article (Journal Article)

Magnetic resonance imaging has been used to visualize size-based protein separations inside operating chromatography columns. The effects of flow nonuniformity have been observed and analyzed quantitatively through concentration profiles of tracers measured inside the column. Analysis of these profiles provides local and averaged intracolumn plate height values for characterization of dispersion and flow nonuniformity. The magnetic resonance measurements compare favorably with conventional chromatographic measurements of column efficiency and provide more detailed insights into nonideal column performance.

Full Text

Duke Authors

Cited Authors

  • Mitchell, NS; Hagel, L; Fernandez, EJ

Published Date

  • August 29, 1997

Published In

Volume / Issue

  • 779 / 1-2

Start / End Page

  • 73 - 89

PubMed ID

  • 9335119

International Standard Serial Number (ISSN)

  • 0021-9673

Digital Object Identifier (DOI)

  • 10.1016/s0021-9673(97)00457-3


  • eng

Conference Location

  • Netherlands