Synthesis of Cationic Amphiphilic Surface-Block Polyester Dendrimers

Published

Journal Article

© 2017, Springer Science+Business Media, LLC. Cationic amphiphilic polyester dendrimers possessing varied numbers of choline and alkyl chains on the periphery were synthesized and characterized. A combination of divergent and convergent synthetic routes was used to efficiently prepare the dendrimers. All of the amphiphiles bound DNA as determined by an ethidium bromide displacement assay, and the dendrimer that contained two choline and four alkyl chains exhibited the smallest charge ratio. Only this amphiphilic dendrimer formed a well-defined structure alone or with DNA in solution, while the other dendrimer compositions gave aggregates. Specifically, small vesicular structures of several 100 nanometers in diameter were observed with DNA, and this dendrimerplex exhibited the greatest transfection efficiency of the group. The results of this study highlight the important role that charge, hydrophobicity, size, and compaction ability play in binding and formation of DNA-dendrimer complexes and the resulting transfection efficiency. Graphical Abstract: Cationic amphiphilic polyester dendrimers possessing different numbers of choline heads groups and myristic acid alkyl chains were synthesized and evaluated for gene transfection. [Figure not available: see fulltext.].

Full Text

Duke Authors

Cited Authors

  • Prata, CAH; Luman, NR; Li, Y; Luo, D; McIntosh, TJ; Barthélémy, P; Grinstaff, MW

Published Date

  • March 1, 2018

Published In

Volume / Issue

  • 28 / 2

Start / End Page

  • 383 - 398

Electronic International Standard Serial Number (EISSN)

  • 1574-1451

International Standard Serial Number (ISSN)

  • 1574-1443

Digital Object Identifier (DOI)

  • 10.1007/s10904-017-0651-4

Citation Source

  • Scopus