Methods to Visualize MAVS Subcellular Localization.

Published

Journal Article

The mitochondrial antiviral signaling (MAVS) protein is a central adaptor protein required for antiviral innate immune signaling. To facilitate its roles in innate immunity, MAVS localizes to multiple intracellular membranous compartments, including the mitochondria, the mitochondrial-associated ER membrane (MAM), and peroxisomes. Studies of MAVS function therefore often require an analysis of MAVS localization. To detect MAVS protein on intracellular membranes, biochemical fractionation to isolate MAMs, mitochondria, or peroxisomes can be used. Further, immunofluorescence with antibodies against specific membrane markers can be used to visualize MAVS distribution throughout the cell. Here, we describe the biochemical fractionation and immunofluorescence protocols used to detect MAVS subcellular localization.

Full Text

Duke Authors

Cited Authors

  • Vazquez, C; Beachboard, DC; Horner, SM

Published Date

  • January 2017

Published In

Volume / Issue

  • 1656 /

Start / End Page

  • 131 - 142

PubMed ID

  • 28808966

Pubmed Central ID

  • 28808966

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-7237-1_7

Language

  • eng