Current technology for the derivation of pluripotent stem cell lines from human embryos.
Journal Article (Journal Article)
Technology for the derivation, propagation, and characterization of pluripotent stem cell lines from the human embryo has undergone considerable refinement and improvement since the first published description of human embryonic stem cells in 1998. In particular, there has been extensive effort to optimize protocols and develop defined culture systems with a view toward future clinical applications of embryonic stem cell-derived products. Here, we review the current status of methodology for human embryonic stem cell derivation and culture, and we highlight the challenges that remain for workers in the field.
Full Text
Duke Authors
Cited Authors
- Hasegawa, K; Pomeroy, JE; Pera, MF
Published Date
- June 4, 2010
Published In
Volume / Issue
- 6 / 6
Start / End Page
- 521 - 531
PubMed ID
- 20569689
Electronic International Standard Serial Number (EISSN)
- 1875-9777
Digital Object Identifier (DOI)
- 10.1016/j.stem.2010.05.010
Language
- eng
Conference Location
- United States