Adhesion mediated by bonds in series.

Published

Journal Article

Cell adhesion in physiological situations and biotechnological applications is often mediated through serial protein/protein linkages. The adhesion strength of cell/substrate contacts through receptor/ligand bonds in series is explored with a simple mathematical model and quantified with an experimental adhesion assay. A deterministic, mass-action model is developed to describe the attachment and detachment of cell/substrate contacts through single and serial bonds. The experimental system is comprised of protein-coated beads, soluble antibody linkers, and an antibody-coated glass surface. Using the Radial-Flow Detachment Assay, the adhesion strengths of bead/substrate contacts through single and serial linkages are measured. Taken together, this work shows that the specific adhesion strength of the cell/substrate contacts comprised of two receptor/ligand bonds in series is less than the specific adhesion strength of the cell/substrate contacts comprised of either of the bonds separately. In addition, the force to rupture contacts comprised of bonds in series varies with the concentration of the solution linker. The model predicts that the locus of linkage fracture location has only a mild dependence on the ratio of relative bond affinities.

Full Text

Duke Authors

Cited Authors

  • Saterbak, A; Lauffenburger, DA

Published Date

  • September 1996

Published In

Volume / Issue

  • 12 / 5

Start / End Page

  • 682 - 699

PubMed ID

  • 8879156

Pubmed Central ID

  • 8879156

Electronic International Standard Serial Number (EISSN)

  • 1520-6033

International Standard Serial Number (ISSN)

  • 8756-7938

Digital Object Identifier (DOI)

  • 10.1021/bp960061u

Language

  • eng