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Chondroitin Sulfate Inhibits Monocyte Chemoattractant Protein-1 Release From 3T3-L1 Adipocytes: A New Treatment Opportunity for Obesity-Related Inflammation?

Publication ,  Conference
Stabler, TV; Montell, E; Vergés, J; Huebner, JL; Kraus, VB
Published in: Biomarker Insights
August 22, 2017

Monocyte chemoattractant protein-1 (MCP-1) overproduction from inflamed adipose tissue is a major contributor to obesity-related metabolic syndromes. 3T3-L1 embryonic fibroblasts were cultured and differentiated into adipocytes using an established protocol. Adipocytes were treated with lipopolysaccharide (LPS) to induce inflammation and thus MCP-1 release. At the same time, varying concentrations of chondroitin sulfate (CS) were added in a physiologically relevant range (10-200 µg/mL) to determine its impact on MCP-1 release. Chondroitin sulfate, a natural glycosaminoglycan of connective tissue including the cartilage extracellular matrix, was chosen on the basis of our previous studies demonstrating its anti-inflammatory effect on macrophages. Because the main action of MCP-1 is to induce monocyte migration, cultured THP-1 monocytes were used to test whether CS at the highest physiologically relevant concentration could inhibit cell migration induced by human recombinant MCP-1. Chondroitin sulfate (100-200 µg/mL) inhibited MCP-1 release from inflamed adipocytes in a dose-dependent manner (P <.01, 95% confidence interval [CI]: −5.89 to −3.858 at 100 µg/mL and P <.001, 95% CI: −6.028 to −3.996 at 200 µg/mL) but had no effect on MCP-1–driven chemotaxis of THP-1 monocytes. In summary, CS could be expected to reduce macrophage infiltration into adipose tissue by reduction in adipocyte expression and release of MCP-1 and as such might reduce adipose tissue inflammation in response to pro-inflammatory stimuli such as LPS, now increasingly recognized to be relevant in vivo.

Duke Scholars

Published In

Biomarker Insights

DOI

EISSN

1177-2719

Publication Date

August 22, 2017

Volume

12

Related Subject Headings

  • 3214 Pharmacology and pharmaceutical sciences
  • 3205 Medical biochemistry and metabolomics
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 1101 Medical Biochemistry and Metabolomics
 

Citation

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Stabler, T. V., Montell, E., Vergés, J., Huebner, J. L., & Kraus, V. B. (2017). Chondroitin Sulfate Inhibits Monocyte Chemoattractant Protein-1 Release From 3T3-L1 Adipocytes: A New Treatment Opportunity for Obesity-Related Inflammation? In Biomarker Insights (Vol. 12). https://doi.org/10.1177/1177271917726964
Stabler, T. V., E. Montell, J. Vergés, J. L. Huebner, and V. B. Kraus. “Chondroitin Sulfate Inhibits Monocyte Chemoattractant Protein-1 Release From 3T3-L1 Adipocytes: A New Treatment Opportunity for Obesity-Related Inflammation?” In Biomarker Insights, Vol. 12, 2017. https://doi.org/10.1177/1177271917726964.

Published In

Biomarker Insights

DOI

EISSN

1177-2719

Publication Date

August 22, 2017

Volume

12

Related Subject Headings

  • 3214 Pharmacology and pharmaceutical sciences
  • 3205 Medical biochemistry and metabolomics
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 1101 Medical Biochemistry and Metabolomics