A comparison of two catalase preparations used to examine vascular permeability.

Published

Journal Article

Beef liver catalase has been used as a tracer in cytochemical studies of vascular permeability. The use of Sigma catalase C-40 and C-100 preparations in capillary permeability studies of the ileojejunum and choriocapillaris was examined. Catalase C-40 is restricted by the fenestrated capillaries of the ileojejunum in contrast to their permeability to C-100. The choriocapillaris restricts both catalase preparations. Sephadex G-200 chromatography of plasma samples incubated with catalase C-40, or from C-40-injected animals, demonstrated an increase in the molecular weight of the tracer. No increase in molecular weight was evident for catalase C-100. The isoelectric point of both preparations was 5.4-5.7. These findings indicate that Sigma catalase C-100 is the preferred preparation for use in vascular permeability studies.

Full Text

Cited Authors

  • Hart, TK; Pino, RM

Published Date

  • May 1, 1985

Published In

Volume / Issue

  • 29 / 3

Start / End Page

  • 412 - 420

PubMed ID

  • 3999995

Pubmed Central ID

  • 3999995

Electronic International Standard Serial Number (EISSN)

  • 1095-9319

International Standard Serial Number (ISSN)

  • 0026-2862

Digital Object Identifier (DOI)

  • 10.1016/0026-2862(85)90029-9

Language

  • eng