Isolation of collagen from cortical bovine bone for preparation of porous collagen sponges


Conference Paper

Fabrication of collagen sponges from type I collagen isolated from cortical bovine femur bone is reported. Collagen was extracted with 0.1 M EDTA by refreshing the solution every 24 h for 9 days. Sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE) demonstrated the isolation of intact pure type I collagen. The sponges were fabricated by freeze-drying and heat-dehydration cross-linking of collagen solution. The resulting sponges showed fibrils arranged in a highly porous network. These sponges can be used for tissue engineering as well as for bone-like composite fabrication. © The Society for Experimental Mechanics, Inc. 2013.

Full Text

Duke Authors

Cited Authors

  • Castro-Ceseña, AB; Novitskaya, EE; Phadke, A; Varghese, S; McKittrick, J

Published Date

  • January 1, 2013

Published In

Volume / Issue

  • 5 /

Start / End Page

  • 73 - 78

Electronic International Standard Serial Number (EISSN)

  • 2191-5652

International Standard Serial Number (ISSN)

  • 2191-5644

International Standard Book Number 13 (ISBN-13)

  • 9781461444268

Digital Object Identifier (DOI)

  • 10.1007/978-1-4614-4427-5_11

Citation Source

  • Scopus