Mineralized synthetic matrices as an instructive microenvironment for osteogenic differentiation of human mesenchymal stem cells.

Published

Journal Article

The effect of substrate-mediated signals on osteogenic differentiation of hMSCs is studied using a synthetic bone-like material comprising both organic and inorganic components that supports adhesion, spreading, and proliferation of hMSCs. hMSCs undergo osteogenic differentiation even in the absence of osteogenesis-inducing supplements. They exhibit higher expressions of Runx2, BSP, and OCN compared to their matrix-rigidity-matched, non-mineralized hydrogel counterparts. The mineralized-hydrogel-assisted osteogenic differentiation of hMSCs could be attributed to their exposure to high local concentrations of calcium and phosphate ions in conjunction with chemical and topological cues arising from the hydrogel-bound calcium phosphate mineral layer.

Full Text

Duke Authors

Cited Authors

  • Phadke, A; Shih, Y-RV; Varghese, S

Published Date

  • August 2012

Published In

Volume / Issue

  • 12 / 8

Start / End Page

  • 1022 - 1032

PubMed ID

  • 22760917

Pubmed Central ID

  • 22760917

Electronic International Standard Serial Number (EISSN)

  • 1616-5195

Digital Object Identifier (DOI)

  • 10.1002/mabi.201100289

Language

  • eng

Conference Location

  • Germany