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Mineralized synthetic matrices as an instructive microenvironment for osteogenic differentiation of human mesenchymal stem cells.

Publication ,  Journal Article
Phadke, A; Shih, Y-RV; Varghese, S
Published in: Macromol Biosci
August 2012

The effect of substrate-mediated signals on osteogenic differentiation of hMSCs is studied using a synthetic bone-like material comprising both organic and inorganic components that supports adhesion, spreading, and proliferation of hMSCs. hMSCs undergo osteogenic differentiation even in the absence of osteogenesis-inducing supplements. They exhibit higher expressions of Runx2, BSP, and OCN compared to their matrix-rigidity-matched, non-mineralized hydrogel counterparts. The mineralized-hydrogel-assisted osteogenic differentiation of hMSCs could be attributed to their exposure to high local concentrations of calcium and phosphate ions in conjunction with chemical and topological cues arising from the hydrogel-bound calcium phosphate mineral layer.

Duke Scholars

Published In

Macromol Biosci

DOI

EISSN

1616-5195

Publication Date

August 2012

Volume

12

Issue

8

Start / End Page

1022 / 1032

Location

Germany

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Polymers
  • Osteogenesis
  • Osteoblasts
  • Mesenchymal Stem Cells
  • Integrin-Binding Sialoprotein
  • Hydrogels
  • Humans
  • Core Binding Factor Alpha 1 Subunit
 

Citation

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Phadke, A., Shih, Y.-R., & Varghese, S. (2012). Mineralized synthetic matrices as an instructive microenvironment for osteogenic differentiation of human mesenchymal stem cells. Macromol Biosci, 12(8), 1022–1032. https://doi.org/10.1002/mabi.201100289
Phadke, Ameya, Yu-Ru V. Shih, and Shyni Varghese. “Mineralized synthetic matrices as an instructive microenvironment for osteogenic differentiation of human mesenchymal stem cells.Macromol Biosci 12, no. 8 (August 2012): 1022–32. https://doi.org/10.1002/mabi.201100289.
Phadke, Ameya, et al. “Mineralized synthetic matrices as an instructive microenvironment for osteogenic differentiation of human mesenchymal stem cells.Macromol Biosci, vol. 12, no. 8, Aug. 2012, pp. 1022–32. Pubmed, doi:10.1002/mabi.201100289.
Journal cover image

Published In

Macromol Biosci

DOI

EISSN

1616-5195

Publication Date

August 2012

Volume

12

Issue

8

Start / End Page

1022 / 1032

Location

Germany

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Polymers
  • Osteogenesis
  • Osteoblasts
  • Mesenchymal Stem Cells
  • Integrin-Binding Sialoprotein
  • Hydrogels
  • Humans
  • Core Binding Factor Alpha 1 Subunit