In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells.
Published
Journal Article
Development of clinically relevant regenerative medicine therapies using human embryonic stem cells (hESCs) requires production of a simple and readily expandable cell population that can be directed to form functional 3D tissue in an in vivo environment. We describe an efficient derivation method and characterization of mesenchymal stem cells (MSCs) from hESCs (hESCd-MSCs) that have multilineage differentiation potential and are capable of producing fat, cartilage, and bone in vitro. Furthermore, we highlight their in vivo survival and commitment to the chondrogenic lineage in a microenvironment comprising chondrocyte-secreted morphogenetic factors and hydrogels. Normal cartilage architecture was established in rat osteochondral defects after treatment with chondrogenically-committed hESCd-MSCs. In view of the limited available cell sources for tissue engineering applications, these embryonic-derived cells show significant potential in musculoskeletal tissue regeneration applications.
Full Text
Duke Authors
Cited Authors
- Hwang, NS; Varghese, S; Lee, HJ; Zhang, Z; Ye, Z; Bae, J; Cheng, L; Elisseeff, J
Published Date
- December 30, 2008
Published In
Volume / Issue
- 105 / 52
Start / End Page
- 20641 - 20646
PubMed ID
- 19095799
Pubmed Central ID
- 19095799
Electronic International Standard Serial Number (EISSN)
- 1091-6490
Digital Object Identifier (DOI)
- 10.1073/pnas.0809680106
Language
- eng
Conference Location
- United States