Nitrous oxide vibrational energy relaxation is a probe of interfacial water in lipid bilayers

Journal Article (Journal Article)

Ultrafast infrared spectroscopy of N2O is shown to be a sensitive probe of hydrophobic and aqueous sites in lipid bilayers. Distinct rates of VER of the ν3 antisymmetric stretching mode of N 2O can be distinguished for N2O solvated in the acyl tail, interfacial water, and bulk water regions of hydrated dioleoylphosphatidylcholine (DOPC) bilayers. The lifetime of the interfacial N2O population is hydration-dependent. This effect is attributed to changes in the density of intermolecular states resonant with the ν3 band (∼2230 cm-1) resulting from oriented interfacial water molecules near the lipid phosphate. Thus, the N2O VER rate becomes a novel and experimentally convenient tool for reporting on the structure and dynamics of interfacial water in lipids and, potentially, in other biological systems. © 2008 American Chemical Society.

Full Text

Duke Authors

Cited Authors

  • Chieffo, LR; Shattuck, JT; Pinnick, E; Amsden, JJ; Hong, MK; Wang, F; Erramilli, S; Ziegler, LD

Published Date

  • October 9, 2008

Published In

Volume / Issue

  • 112 / 40

Start / End Page

  • 12776 - 12782

International Standard Serial Number (ISSN)

  • 1520-6106

Digital Object Identifier (DOI)

  • 10.1021/jp8012283

Citation Source

  • Scopus