Effects of raltegravir on 2-long terminal repeat circle junctions in HIV type 1 viremic and aviremic patients.

Published

Journal Article

Although 2-long terminal repeat (2-LTR) circles are only a fraction of the total viral DNA in infected cells, sequence analysis of 2-LTR circles reveals critical information regarding viral DNA synthesis and the nature of actively replicating virus. It was observed that a large proportion of the 2-LTR circular molecules in the peripheral blood mononuclear cell (PBMC) DNA of infected individuals are mutated at the circle junction. The integrase inhibitor raltegravir (RAL) blocks the strand transfer step of the integration of HIV-1; as a consequence of abortive integration a significant increase of episomal 2-LTR circles is observed. Moreover, it was demonstrated that in patients treated with highly active retroviral therapy (HAART) changes in 2-LTR concentration did not affect junction sequences and flanking regions of 2-LTR. Here we evaluated whether RAL therapy could have a differential impact on the 2-LTR circle junctional sequences in patients with different virological profiles at the time of starting RAL therapy. Sequence analysis indicates that RAL acts differently in the two populations.

Full Text

Duke Authors

Cited Authors

  • Bona, R; Baroncelli, S; D'Ettorre, G; Andreotti, M; Ceccarelli, G; Filati, P; Leone, P; Blasi, M; Michelini, Z; Galluzzo, CM; Mallano, A; Vullo, V; Cara, A

Published Date

  • October 2013

Published In

Volume / Issue

  • 29 / 10

Start / End Page

  • 1365 - 1369

PubMed ID

  • 23802629

Pubmed Central ID

  • 23802629

Electronic International Standard Serial Number (EISSN)

  • 1931-8405

International Standard Serial Number (ISSN)

  • 0889-2229

Digital Object Identifier (DOI)

  • 10.1089/aid.2013.0047

Language

  • eng