Neuregulin-induced expression of the acetylcholine receptor requires endocytosis of ErbB receptors.

Published

Journal Article

Neuregulin-induced expression of the acetylcholine receptor (AChR) contributes to high concentration of the receptor at the neuromuscular junction (NMJ). Neuregulin-1 activates ErbB tyrosine kinases and subsequently intracellular kinases including Erk that is required for induced AChR expression. Recent studies demonstrate that ligand-induced internalization may regulate signaling of various receptor tyrosine kinases. However, the role of induced ErbB endocytosis in regulating AChR expression was unclear. Here we provide evidence that ErbB tyrosine kinases became rapidly internalized in response to neuregulin. The internalization required the kinase activity of ErbB proteins and involved a clathrin-dependent endocytic pathway. Moreover, neuregulin-induced Erk activation and AChR expression were attenuated when ErbB endocytosis was blocked. These results indicate that ErbB proteins undergo endocytosis in response to neuregulin, and this process is required for neuregulin signaling and induced AChR expression.

Full Text

Duke Authors

Cited Authors

  • Yang, X-L; Huang, YZ; Xiong, WC; Mei, L

Published Date

  • February 2005

Published In

Volume / Issue

  • 28 / 2

Start / End Page

  • 335 - 346

PubMed ID

  • 15691714

Pubmed Central ID

  • 15691714

International Standard Serial Number (ISSN)

  • 1044-7431

Digital Object Identifier (DOI)

  • 10.1016/j.mcn.2004.10.001

Language

  • eng

Conference Location

  • United States