Imaging Cytometry of Human Leukocytes with Third Harmonic Generation Microscopy.

Journal Article (Journal Article)

Based on third-harmonic-generation (THG) microscopy and a k-means clustering algorithm, we developed a label-free imaging cytometry method to differentiate and determine the types of human leukocytes. According to the size and average intensity of cells in THG images, in a two-dimensional scatter plot, the neutrophils, monocytes, and lymphocytes in peripheral blood samples from healthy volunteers were clustered into three differentiable groups. Using these features in THG images, we could count the number of each of the three leukocyte types both in vitro and in vivo. The THG imaging-based counting results agreed well with conventional blood count results. In the future, we believe that the combination of this THG microscopy-based imaging cytometry approach with advanced texture analysis of sub-cellular features can differentiate and count more types of blood cells with smaller quantities of blood.

Full Text

Duke Authors

Cited Authors

  • Wu, C-H; Wang, T-D; Hsieh, C-H; Huang, S-H; Lin, J-W; Hsu, S-C; Wu, H-T; Wu, Y-M; Liu, T-M

Published Date

  • November 2016

Published In

Volume / Issue

  • 6 /

Start / End Page

  • 37210 -

PubMed ID

  • 27845443

Pubmed Central ID

  • PMC5109028

Electronic International Standard Serial Number (EISSN)

  • 2045-2322

International Standard Serial Number (ISSN)

  • 2045-2322

Digital Object Identifier (DOI)

  • 10.1038/srep37210


  • eng