Reconciling conflicting models for global control of cell-cycle transcription.

Published

Journal Article

Models for the control of global cell-cycle transcription have advanced from a CDK-APC/C oscillator, a transcription factor (TF) network, to coupled CDK-APC/C and TF networks. Nonetheless, current models were challenged by a recent study that concluded that the cell-cycle transcriptional program is primarily controlled by a CDK-APC/C oscillator in budding yeast. Here we report an analysis of the transcriptome dynamics in cyclin mutant cells that were not queried in the previous study. We find that B-cyclin oscillation is not essential for control of phase-specific transcription. Using a mathematical model, we demonstrate that the function of network TFs can be retained in the face of significant reductions in transcript levels. Finally, we show that cells arrested at mitotic exit with non-oscillating levels of B-cyclins continue to cycle transcriptionally. Taken together, these findings support a critical role of a TF network and a requirement for CDK activities that need not be periodic.

Full Text

Duke Authors

Cited Authors

  • Cho, C-Y; Motta, FC; Kelliher, CM; Deckard, A; Haase, SB

Published Date

  • October 2017

Published In

Volume / Issue

  • 16 / 20

Start / End Page

  • 1965 - 1978

PubMed ID

  • 28934013

Pubmed Central ID

  • 28934013

Electronic International Standard Serial Number (EISSN)

  • 1551-4005

International Standard Serial Number (ISSN)

  • 1538-4101

Digital Object Identifier (DOI)

  • 10.1080/15384101.2017.1367073

Language

  • eng