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Isolation of exosomes from whole blood by integrating acoustics and microfluidics.

Publication ,  Journal Article
Wu, M; Ouyang, Y; Wang, Z; Zhang, R; Huang, P-H; Chen, C; Li, H; Li, P; Quinn, D; Dao, M; Suresh, S; Sadovsky, Y; Huang, TJ
Published in: Proceedings of the National Academy of Sciences of the United States of America
October 2017

Exosomes are nanoscale extracellular vesicles that play an important role in many biological processes, including intercellular communications, antigen presentation, and the transport of proteins, RNA, and other molecules. Recently there has been significant interest in exosome-related fundamental research, seeking new exosome-based biomarkers for health monitoring and disease diagnoses. Here, we report a separation method based on acoustofluidics (i.e., the integration of acoustics and microfluidics) to isolate exosomes directly from whole blood in a label-free and contact-free manner. This acoustofluidic platform consists of two modules: a microscale cell-removal module that first removes larger blood components, followed by extracellular vesicle subgroup separation in the exosome-isolation module. In the cell-removal module, we demonstrate the isolation of 110-nm particles from a mixture of micro- and nanosized particles with a yield greater than 99%. In the exosome-isolation module, we isolate exosomes from an extracellular vesicle mixture with a purity of 98.4%. Integrating the two acoustofluidic modules onto a single chip, we isolated exosomes from whole blood with a blood cell removal rate of over 99.999%. With its ability to perform rapid, biocompatible, label-free, contact-free, and continuous-flow exosome isolation, the integrated acoustofluidic device offers a unique approach to investigate the role of exosomes in the onset and progression of human diseases with potential applications in health monitoring, medical diagnosis, targeted drug delivery, and personalized medicine.

Duke Scholars

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Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

October 2017

Volume

114

Issue

40

Start / End Page

10584 / 10589

Related Subject Headings

  • Microfluidic Analytical Techniques
  • Exosomes
  • Blood Cells
  • Acoustics
 

Citation

APA
Chicago
ICMJE
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Wu, M., Ouyang, Y., Wang, Z., Zhang, R., Huang, P.-H., Chen, C., … Huang, T. J. (2017). Isolation of exosomes from whole blood by integrating acoustics and microfluidics. Proceedings of the National Academy of Sciences of the United States of America, 114(40), 10584–10589. https://doi.org/10.1073/pnas.1709210114
Wu, Mengxi, Yingshi Ouyang, Zeyu Wang, Rui Zhang, Po-Hsun Huang, Chuyi Chen, Hui Li, et al. “Isolation of exosomes from whole blood by integrating acoustics and microfluidics.Proceedings of the National Academy of Sciences of the United States of America 114, no. 40 (October 2017): 10584–89. https://doi.org/10.1073/pnas.1709210114.
Wu M, Ouyang Y, Wang Z, Zhang R, Huang P-H, Chen C, et al. Isolation of exosomes from whole blood by integrating acoustics and microfluidics. Proceedings of the National Academy of Sciences of the United States of America. 2017 Oct;114(40):10584–9.
Wu, Mengxi, et al. “Isolation of exosomes from whole blood by integrating acoustics and microfluidics.Proceedings of the National Academy of Sciences of the United States of America, vol. 114, no. 40, Oct. 2017, pp. 10584–89. Epmc, doi:10.1073/pnas.1709210114.
Wu M, Ouyang Y, Wang Z, Zhang R, Huang P-H, Chen C, Li H, Li P, Quinn D, Dao M, Suresh S, Sadovsky Y, Huang TJ. Isolation of exosomes from whole blood by integrating acoustics and microfluidics. Proceedings of the National Academy of Sciences of the United States of America. 2017 Oct;114(40):10584–10589.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

October 2017

Volume

114

Issue

40

Start / End Page

10584 / 10589

Related Subject Headings

  • Microfluidic Analytical Techniques
  • Exosomes
  • Blood Cells
  • Acoustics