Impact of human CA8 on thermal antinociception in relation to morphine equivalence in mice.
Journal Article (Journal Article)
Recently, we showed that murine dorsal root ganglion (DRG) Car8 expression is a cis-regulated eQTL that determines analgesic responses. In this report, we show that transduction through sciatic nerve injection of DRG with human wild-type carbonic anhydrase-8 using adeno-associated virus viral particles (AAV8-V5-CA8WT) produces analgesia in naive male C57BL/6J mice and antihyperalgesia after carrageenan treatment. A peak mean increase of about 4 s in thermal hindpaw withdrawal latency equaled increases in thermal withdrawal latency produced by 10 mg/kg intraperitoneal morphine in these mice. Allometric conversion of this intraperitoneal morphine dose in mice equals an oral morphine dose of about 146 mg in a 60-kg adult. Our work quantifies for the first time analgesia and antihyperalgesia in an inflammatory pain model after DRG transduction by CA8 gene therapy.
- Fu, ES; Erasso, DM; Zhuang, GZ; Upadhyay, U; Ozdemir, M; Wiltshire, T; Sarantopoulos, KD; Smith, SB; Maixner, W; Martin, ER; Levitt, RC
- December 13, 2017
Volume / Issue
- 28 / 18
Start / End Page
- 1215 - 1220
Pubmed Central ID
Electronic International Standard Serial Number (EISSN)
Digital Object Identifier (DOI)