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Characterizing differences between MSCs and TM cells: Toward autologous stem cell therapies for the glaucomatous trabecular meshwork.

Publication ,  Journal Article
Snider, EJ; Vannatta, RT; Schildmeyer, L; Stamer, WD; Ethier, CR
Published in: J Tissue Eng Regen Med
March 2018

Glaucoma, a leading cause of blindness, is characterized by an increase in intraocular pressure, which is largely determined by resistance to aqueous humour outflow through the trabecular meshwork (TM). In glaucoma, the cellularity of the TM is decreased, and, as a result, stem cell therapies for the TM represent a potential therapeutic option for restoring TM function and treating glaucoma patients. We here focus on adipose derived mesenchymal stem cells (MSCs) as a potential autologous cell source for TM regenerative medicine applications and describe characterization techniques at the messenger (reverse transcription-quantitative polymerase chain reaction), protein (western blotting, flow cytometry), and functional (contractility, phagocytosis) levels to distinguish MSCs from TM cells. We present a panel of 12 transcripts to allow: (a) suitable normalization of reverse transcription-quantitative polymerase chain reaction results across cell types and after exposure to potential differentiation stimuli; (b) distinguishing MSCs from TM cells; (c) distinguishing subtypes of TM cells; and (d) distinguishing TM cells from those in neighbouring tissue. At the protein level, dexamethasone induction of myocilin was a robust discriminating factor between MSCs and TM cells and was complemented by other protein markers. Finally, we show that contractility and phagocytosis differ between MSCs and TM cells. These methods are recommended for use in future differentiation studies to fully define if a functional TM-like phenotype is being achieved.

Duke Scholars

Published In

J Tissue Eng Regen Med

DOI

EISSN

1932-7005

Publication Date

March 2018

Volume

12

Issue

3

Start / End Page

695 / 704

Location

England

Related Subject Headings

  • Transplantation, Autologous
  • Trabecular Meshwork
  • RNA, Messenger
  • Phagocytosis
  • Mesenchymal Stem Cells
  • Mesenchymal Stem Cell Transplantation
  • Humans
  • Glycoproteins
  • Glaucoma
  • Gene Expression Regulation
 

Citation

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Snider, E. J., Vannatta, R. T., Schildmeyer, L., Stamer, W. D., & Ethier, C. R. (2018). Characterizing differences between MSCs and TM cells: Toward autologous stem cell therapies for the glaucomatous trabecular meshwork. J Tissue Eng Regen Med, 12(3), 695–704. https://doi.org/10.1002/term.2488
Snider, Eric J., R Taylor Vannatta, Lisa Schildmeyer, W Daniel Stamer, and C Ross Ethier. “Characterizing differences between MSCs and TM cells: Toward autologous stem cell therapies for the glaucomatous trabecular meshwork.J Tissue Eng Regen Med 12, no. 3 (March 2018): 695–704. https://doi.org/10.1002/term.2488.
Snider EJ, Vannatta RT, Schildmeyer L, Stamer WD, Ethier CR. Characterizing differences between MSCs and TM cells: Toward autologous stem cell therapies for the glaucomatous trabecular meshwork. J Tissue Eng Regen Med. 2018 Mar;12(3):695–704.
Snider, Eric J., et al. “Characterizing differences between MSCs and TM cells: Toward autologous stem cell therapies for the glaucomatous trabecular meshwork.J Tissue Eng Regen Med, vol. 12, no. 3, Mar. 2018, pp. 695–704. Pubmed, doi:10.1002/term.2488.
Snider EJ, Vannatta RT, Schildmeyer L, Stamer WD, Ethier CR. Characterizing differences between MSCs and TM cells: Toward autologous stem cell therapies for the glaucomatous trabecular meshwork. J Tissue Eng Regen Med. 2018 Mar;12(3):695–704.
Journal cover image

Published In

J Tissue Eng Regen Med

DOI

EISSN

1932-7005

Publication Date

March 2018

Volume

12

Issue

3

Start / End Page

695 / 704

Location

England

Related Subject Headings

  • Transplantation, Autologous
  • Trabecular Meshwork
  • RNA, Messenger
  • Phagocytosis
  • Mesenchymal Stem Cells
  • Mesenchymal Stem Cell Transplantation
  • Humans
  • Glycoproteins
  • Glaucoma
  • Gene Expression Regulation