Lessons from single-cell transcriptome analysis of oxygen-sensing cells.

Journal Article (Journal Article;Review)

The advent of single-cell RNA-sequencing (RNA-Seq) technology has enabled transcriptome profiling of individual cells. Comprehensive gene expression analysis at the single-cell level has proven to be effective in characterizing the most fundamental aspects of cellular function and identity. This unbiased approach is revolutionary for small and/or heterogeneous tissues like oxygen-sensing cells in identifying key molecules. Here, we review the major methods of current single-cell RNA-Seq technology. We discuss how this technology has advanced the understanding of oxygen-sensing glomus cells in the carotid body and helped uncover novel oxygen-sensing cells and mechanisms in the mice olfactory system. We conclude by providing our perspective on future single-cell RNA-Seq research directed at oxygen-sensing cells.

Full Text

Duke Authors

Cited Authors

  • Zhou, T; Matsunami, H

Published Date

  • May 2018

Published In

Volume / Issue

  • 372 / 2

Start / End Page

  • 403 - 415

PubMed ID

  • 28887696

Pubmed Central ID

  • PMC5843501

Electronic International Standard Serial Number (EISSN)

  • 1432-0878

Digital Object Identifier (DOI)

  • 10.1007/s00441-017-2682-0


  • eng

Conference Location

  • Germany