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A high-throughput acoustic cell sorter.

Publication ,  Journal Article
Ren, L; Chen, Y; Li, P; Mao, Z; Huang, P-H; Rufo, J; Guo, F; Wang, L; McCoy, JP; Levine, SJ; Huang, TJ
Published in: Lab on a chip
October 2015

Acoustic-based fluorescence activated cell sorters (FACS) have drawn increased attention in recent years due to their versatility, high biocompatibility, high controllability, and simple design. However, the sorting throughput for existing acoustic cell sorters is far from optimum for practical applications. Here we report a high-throughput cell sorting method based on standing surface acoustic waves (SSAWs). We utilized a pair of focused interdigital transducers (FIDTs) to generate SSAW with high resolution and high energy efficiency. As a result, the sorting throughput is improved significantly from conventional acoustic-based cell sorting methods. We demonstrated the successful sorting of 10 μm polystyrene particles with a minimum actuation time of 72 μs, which translates to a potential sorting rate of more than 13,800 events per second. Without using a cell-detection unit, we were able to demonstrate an actual sorting throughput of 3300 events per second. Our sorting method can be conveniently integrated with upstream detection units, and it represents an important development towards a functional acoustic-based FACS system.

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Published In

Lab on a chip

DOI

EISSN

1473-0189

ISSN

1473-0197

Publication Date

October 2015

Volume

15

Issue

19

Start / End Page

3870 / 3879

Related Subject Headings

  • Sound
  • Humans
  • Hela Cells
  • HeLa Cells
  • Flow Cytometry
  • Cell Separation
  • Analytical Chemistry
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
 

Citation

APA
Chicago
ICMJE
MLA
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Ren, L., Chen, Y., Li, P., Mao, Z., Huang, P.-H., Rufo, J., … Huang, T. J. (2015). A high-throughput acoustic cell sorter. Lab on a Chip, 15(19), 3870–3879. https://doi.org/10.1039/c5lc00706b
Ren, Liqiang, Yuchao Chen, Peng Li, Zhangming Mao, Po-Hsun Huang, Joseph Rufo, Feng Guo, et al. “A high-throughput acoustic cell sorter.Lab on a Chip 15, no. 19 (October 2015): 3870–79. https://doi.org/10.1039/c5lc00706b.
Ren L, Chen Y, Li P, Mao Z, Huang P-H, Rufo J, et al. A high-throughput acoustic cell sorter. Lab on a chip. 2015 Oct;15(19):3870–9.
Ren, Liqiang, et al. “A high-throughput acoustic cell sorter.Lab on a Chip, vol. 15, no. 19, Oct. 2015, pp. 3870–79. Epmc, doi:10.1039/c5lc00706b.
Ren L, Chen Y, Li P, Mao Z, Huang P-H, Rufo J, Guo F, Wang L, McCoy JP, Levine SJ, Huang TJ. A high-throughput acoustic cell sorter. Lab on a chip. 2015 Oct;15(19):3870–3879.
Journal cover image

Published In

Lab on a chip

DOI

EISSN

1473-0189

ISSN

1473-0197

Publication Date

October 2015

Volume

15

Issue

19

Start / End Page

3870 / 3879

Related Subject Headings

  • Sound
  • Humans
  • Hela Cells
  • HeLa Cells
  • Flow Cytometry
  • Cell Separation
  • Analytical Chemistry
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering