Standing surface acoustic wave (SSAW)-based microfluidic cytometer.

Published

Journal Article

The development of microfluidic chip-based cytometers has become an important area due to their advantages of compact size and low cost. Herein, we demonstrate a sheathless microfluidic cytometer which integrates a standing surface acoustic wave (SSAW)-based microdevice capable of 3D particle/cell focusing with a laser-induced fluorescence (LIF) detection system. Using SSAW, our microfluidic cytometer was able to continuously focus microparticles/cells at the pressure node inside a microchannel. Flow cytometry was successfully demonstrated using this system with a coefficient of variation (CV) of less than 10% at a throughput of ~1000 events s(-1) when calibration beads were used. We also demonstrated that fluorescently labeled human promyelocytic leukemia cells (HL-60) could be effectively focused and detected with our SSAW-based system. This SSAW-based microfluidic cytometer did not require any sheath flows or complex structures, and it allowed for simple operation over a wide range of sample flow rates. Moreover, with the gentle, bio-compatible nature of low-power surface acoustic waves, this technique is expected to be able to preserve the integrity of cells and other bioparticles.

Full Text

Cited Authors

  • Chen, Y; Nawaz, AA; Zhao, Y; Huang, P-H; McCoy, JP; Levine, SJ; Wang, L; Huang, TJ

Published Date

  • March 2014

Published In

Volume / Issue

  • 14 / 5

Start / End Page

  • 916 - 923

PubMed ID

  • 24406848

Pubmed Central ID

  • 24406848

Electronic International Standard Serial Number (EISSN)

  • 1473-0189

International Standard Serial Number (ISSN)

  • 1473-0197

Digital Object Identifier (DOI)

  • 10.1039/c3lc51139a

Language

  • eng