Single-round selection yields a unique retroviral envelope utilizing GPR172A as its host receptor.
Journal Article (Journal Article)
The recognition by a viral envelope of its cognate host-cell receptor is the initial critical step in defining the viral host-range and tissue specificity. This study combines a single-round of selection of a random envelope library with a parallel cDNA screen for receptor function to identify a distinct retroviral envelope/receptor pair. The 11-aa targeting domain of the modified feline leukemia virus envelope consists of a constrained peptide. Critical to the binding of the constrained peptide envelope to its cellular receptor are a pair of internal cysteines and an essential Trp required for maintenance of titers >10(5) lacZ staining units per milliliter. The receptor used for viral entry is the human GPR172A protein, a G-protein-coupled receptor isolated from osteosarcoma cells. The ability to generate unique envelopes capable of using tissue- or disease-specific receptors marks an advance in the development of efficient gene-therapy vectors.
Full Text
Duke Authors
Cited Authors
- Mazari, PM; Linder-Basso, D; Sarangi, A; Chang, Y; Roth, MJ
Published Date
- April 7, 2009
Published In
Volume / Issue
- 106 / 14
Start / End Page
- 5848 - 5853
PubMed ID
- 19307586
Pubmed Central ID
- PMC2667028
Electronic International Standard Serial Number (EISSN)
- 1091-6490
Digital Object Identifier (DOI)
- 10.1073/pnas.0809741106
Language
- eng
Conference Location
- United States