Single-round selection yields a unique retroviral envelope utilizing GPR172A as its host receptor.

Published

Journal Article

The recognition by a viral envelope of its cognate host-cell receptor is the initial critical step in defining the viral host-range and tissue specificity. This study combines a single-round of selection of a random envelope library with a parallel cDNA screen for receptor function to identify a distinct retroviral envelope/receptor pair. The 11-aa targeting domain of the modified feline leukemia virus envelope consists of a constrained peptide. Critical to the binding of the constrained peptide envelope to its cellular receptor are a pair of internal cysteines and an essential Trp required for maintenance of titers >10(5) lacZ staining units per milliliter. The receptor used for viral entry is the human GPR172A protein, a G-protein-coupled receptor isolated from osteosarcoma cells. The ability to generate unique envelopes capable of using tissue- or disease-specific receptors marks an advance in the development of efficient gene-therapy vectors.

Full Text

Duke Authors

Cited Authors

  • Mazari, PM; Linder-Basso, D; Sarangi, A; Chang, Y; Roth, MJ

Published Date

  • April 7, 2009

Published In

Volume / Issue

  • 106 / 14

Start / End Page

  • 5848 - 5853

PubMed ID

  • 19307586

Pubmed Central ID

  • 19307586

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.0809741106

Language

  • eng

Conference Location

  • United States