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An embryonic system to assess direct and indirect Wnt transcriptional targets.

Publication ,  Journal Article
Suresh, J; Harmston, N; Lim, KK; Kaur, P; Jin, HJ; Lusk, JB; Petretto, E; Tolwinski, NS
Published in: Scientific reports
September 2017

During animal development, complex signals determine and organize a vast number of tissues using a very small number of signal transduction pathways. These developmental signaling pathways determine cell fates through a coordinated transcriptional response that remains poorly understood. The Wnt pathway is involved in a variety of these cellular functions, and its signals are transmitted in part through a β-catenin/TCF transcriptional complex. Here we report an in vivo Drosophila assay that can be used to distinguish between activation, de-repression and repression of transcriptional responses, separating upstream and downstream pathway activation and canonical/non-canonical Wnt signals in embryos. We find specific sets of genes downstream of both β-catenin and TCF with an additional group of genes regulated by Wnt, while the non-canonical Wnt4 regulates a separate cohort of genes. We correlate transcriptional changes with phenotypic outcomes of cell differentiation and embryo size, showing our model can be used to characterize developmental signaling compartmentalization in vivo.

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Published In

Scientific reports

DOI

EISSN

2045-2322

ISSN

2045-2322

Publication Date

September 2017

Volume

7

Issue

1

Start / End Page

11092

Related Subject Headings

  • Wnt Signaling Pathway
  • Wnt Proteins
  • Transcriptome
  • Transcriptional Activation
  • Protein Binding
  • Phenotype
  • Mutation
  • Gene Ontology
  • Gene Expression Regulation, Developmental
  • Gene Expression Profiling
 

Citation

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Suresh, J., Harmston, N., Lim, K. K., Kaur, P., Jin, H. J., Lusk, J. B., … Tolwinski, N. S. (2017). An embryonic system to assess direct and indirect Wnt transcriptional targets. Scientific Reports, 7(1), 11092. https://doi.org/10.1038/s41598-017-11519-z
Suresh, Jahnavi, Nathan Harmston, Ka Keat Lim, Prameet Kaur, Helen Jingshu Jin, Jay B. Lusk, Enrico Petretto, and Nicholas S. Tolwinski. “An embryonic system to assess direct and indirect Wnt transcriptional targets.Scientific Reports 7, no. 1 (September 2017): 11092. https://doi.org/10.1038/s41598-017-11519-z.
Suresh J, Harmston N, Lim KK, Kaur P, Jin HJ, Lusk JB, et al. An embryonic system to assess direct and indirect Wnt transcriptional targets. Scientific reports. 2017 Sep;7(1):11092.
Suresh, Jahnavi, et al. “An embryonic system to assess direct and indirect Wnt transcriptional targets.Scientific Reports, vol. 7, no. 1, Sept. 2017, p. 11092. Epmc, doi:10.1038/s41598-017-11519-z.
Suresh J, Harmston N, Lim KK, Kaur P, Jin HJ, Lusk JB, Petretto E, Tolwinski NS. An embryonic system to assess direct and indirect Wnt transcriptional targets. Scientific reports. 2017 Sep;7(1):11092.

Published In

Scientific reports

DOI

EISSN

2045-2322

ISSN

2045-2322

Publication Date

September 2017

Volume

7

Issue

1

Start / End Page

11092

Related Subject Headings

  • Wnt Signaling Pathway
  • Wnt Proteins
  • Transcriptome
  • Transcriptional Activation
  • Protein Binding
  • Phenotype
  • Mutation
  • Gene Ontology
  • Gene Expression Regulation, Developmental
  • Gene Expression Profiling