An embryonic system to assess direct and indirect Wnt transcriptional targets.

Published

Journal Article

During animal development, complex signals determine and organize a vast number of tissues using a very small number of signal transduction pathways. These developmental signaling pathways determine cell fates through a coordinated transcriptional response that remains poorly understood. The Wnt pathway is involved in a variety of these cellular functions, and its signals are transmitted in part through a β-catenin/TCF transcriptional complex. Here we report an in vivo Drosophila assay that can be used to distinguish between activation, de-repression and repression of transcriptional responses, separating upstream and downstream pathway activation and canonical/non-canonical Wnt signals in embryos. We find specific sets of genes downstream of both β-catenin and TCF with an additional group of genes regulated by Wnt, while the non-canonical Wnt4 regulates a separate cohort of genes. We correlate transcriptional changes with phenotypic outcomes of cell differentiation and embryo size, showing our model can be used to characterize developmental signaling compartmentalization in vivo.

Full Text

Duke Authors

Cited Authors

  • Suresh, J; Harmston, N; Lim, KK; Kaur, P; Jin, HJ; Lusk, JB; Petretto, E; Tolwinski, NS

Published Date

  • September 11, 2017

Published In

Volume / Issue

  • 7 / 1

Start / End Page

  • 11092 -

PubMed ID

  • 28894169

Pubmed Central ID

  • 28894169

Electronic International Standard Serial Number (EISSN)

  • 2045-2322

International Standard Serial Number (ISSN)

  • 2045-2322

Digital Object Identifier (DOI)

  • 10.1038/s41598-017-11519-z

Language

  • eng