Framework for gradual progression of cell ontogeny in the Arabidopsis root meristem.

Journal Article

In plants, apical meristems allow continuous growth along the body axis. Within the root apical meristem, a group of slowly dividing quiescent center cells is thought to limit stem cell activity to directly neighboring cells, thus endowing them with unique properties, distinct from displaced daughters. This binary identity of the stem cells stands in apparent contradiction to the more gradual changes in cell division potential and differentiation that occur as cells move further away from the quiescent center. To address this paradox and to infer molecular organization of the root meristem, we used a whole-genome approach to determine dominant transcriptional patterns along root ontogeny zones. We found that the prevalent patterns are expressed in two opposing gradients. One is characterized by genes associated with development, the other enriched in differentiation genes. We confirmed these transcript gradients, and demonstrate that these translate to gradients in protein accumulation and gradual changes in cellular properties. We also show that gradients are genetically controlled through multiple pathways. Based on these findings, we propose that cells in the Arabidopsis root meristem gradually transition from stem cell activity toward differentiation.

Full Text

Duke Authors

Cited Authors

  • Wendrich, JR; Möller, BK; Li, S; Saiga, S; Sozzani, R; Benfey, PN; De Rybel, B; Weijers, D

Published Date

  • October 2, 2017

Published In

Volume / Issue

  • 114 / 42

Start / End Page

  • E8922 - E8929

PubMed ID

  • 28973915

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1707400114

Language

  • eng