A single gp120 residue can affect HIV-1 tropism in macaques.

Published

Journal Article

Species-dependent variation in proteins that aid or limit virus replication determines the ability of lentiviruses to jump between host species. Identifying and overcoming these differences facilitates the development of animal models for HIV-1, including models based on chimeric SIVs that express HIV-1 envelope (Env) glycoproteins, (SHIVs) and simian-tropic HIV-1 (stHIV) strains. Here, we demonstrate that the inherently poor ability of most HIV-1 Env proteins to use macaque CD4 as a receptor is improved during adaptation by virus passage in macaques. We identify a single amino acid, A281, in HIV-1 Env that consistently changes during adaptation in macaques and affects the ability of HIV-1 Env to use macaque CD4. Importantly, mutations at A281 do not markedly affect HIV-1 Env neutralization properties. Our findings should facilitate the design of HIV-1 Env proteins for use in non-human primate models and thus expedite the development of clinically relevant reagents for testing interventions against HIV-1.

Full Text

Duke Authors

Cited Authors

  • Del Prete, GQ; Keele, BF; Fode, J; Thummar, K; Swanstrom, AE; Rodriguez, A; Raymond, A; Estes, JD; LaBranche, CC; Montefiori, DC; KewalRamani, VN; Lifson, JD; Bieniasz, PD; Hatziioannou, T

Published Date

  • September 25, 2017

Published In

Volume / Issue

  • 13 / 9

Start / End Page

  • e1006572 -

PubMed ID

  • 28945790

Pubmed Central ID

  • 28945790

Electronic International Standard Serial Number (EISSN)

  • 1553-7374

International Standard Serial Number (ISSN)

  • 1553-7366

Digital Object Identifier (DOI)

  • 10.1371/journal.ppat.1006572

Language

  • eng