The diagnostic utility of anterior chamber paracentesis with polymerase chain reaction in anterior uveitis.

Journal Article (Journal Article)

PURPOSE: To determine the value of anterior chamber paracentesis with polymerase chain reaction (PCR) in patients with anterior uveitis. DESIGN: Retrospective observational case series. METHODS: SETTING: Bascom Palmer Eye Institute. PATIENT POPULATION: Fifty-three patients with a diagnosis of anterior uveitis who underwent anterior chamber paracentesis with PCR. OBSERVATION PROCEDURES: Anterior chamber paracentesis with PCR of aqueous fluid. MAIN OUTCOME MEASURE: Diagnostic utility and frequency of management change based on anterior chamber paracentesis and PCR. RESULTS: There were 15 of 53 (28%) acute and 35 of 53 (65%) chronic anterior uveitis patients. PCR positivity of herpes simplex virus, cytomegalovirus, varicella zoster virus, and Epstein-Barr virus in our population were 4 of 53 (8%), 1 of 47 (2%), 1 of 35 (3%), and 1 of 18 (6%). Overall, 7 of 53 patients (13%) had a change in management because of PCR results from anterior chamber paracentesis. Four patients encountered paracentesis complications (4/53, 7.5%), 1 with long-term sequelae. CONCLUSIONS: Anterior chamber paracentesis with PCR had a relatively low diagnostic utility and resulted in few management changes in patients with suspected infectious anterior uveitis.

Full Text

Duke Authors

Cited Authors

  • Anwar, Z; Galor, A; Albini, TA; Miller, D; Perez, V; Davis, JL

Published Date

  • May 2013

Published In

Volume / Issue

  • 155 / 5

Start / End Page

  • 781 - 786

PubMed ID

  • 23415597

Electronic International Standard Serial Number (EISSN)

  • 1879-1891

Digital Object Identifier (DOI)

  • 10.1016/j.ajo.2012.12.008


  • eng

Conference Location

  • United States