High-resolution, noninvasive longitudinal live imaging of immune responses.

Published

Journal Article

Intravital imaging emerged as an indispensible tool in biological research, and a variety of imaging techniques have been developed to noninvasively monitor tissues in vivo. However, most of the current techniques lack the resolution to study events at the single-cell level. Although intravital multiphoton microscopy has addressed this limitation, the need for repeated noninvasive access to the same tissue in longitudinal in vivo studies remains largely unmet. We now report on a previously unexplored approach to study immune responses after transplantation of pancreatic islets into the anterior chamber of the mouse eye. This approach enabled (i) longitudinal, noninvasive imaging of transplanted tissues in vivo; (ii) in vivo cytolabeling to assess cellular phenotype and viability in situ; (iii) local intervention by topical application or intraocular injection; and (iv) real-time tracking of infiltrating immune cells in the target tissue.

Full Text

Duke Authors

Cited Authors

  • Abdulreda, MH; Faleo, G; Molano, RD; Lopez-Cabezas, M; Molina, J; Tan, Y; Echeverria, OAR; Zahr-Akrawi, E; Rodriguez-Diaz, R; Edlund, PK; Leibiger, I; Bayer, AL; Perez, V; Ricordi, C; Caicedo, A; Pileggi, A; Berggren, P-O

Published Date

  • August 2011

Published In

Volume / Issue

  • 108 / 31

Start / End Page

  • 12863 - 12868

PubMed ID

  • 21768391

Pubmed Central ID

  • 21768391

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1105002108

Language

  • eng