Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma.

Journal Article (Journal Article)

As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950-Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra- and interlaboratory quality control and method validation. These analyses were performed using nonstandardized laboratory-independent workflows. The consensus locations were also compared with a previous examination of SRM 1950 by the LIPID MAPS consortium. While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.

Full Text

Duke Authors

Cited Authors

  • Bowden, JA; Heckert, A; Ulmer, CZ; Jones, CM; Koelmel, JP; Abdullah, L; Ahonen, L; Alnouti, Y; Armando, AM; Asara, JM; Bamba, T; Barr, JR; Bergquist, J; Borchers, CH; Brandsma, J; Breitkopf, SB; Cajka, T; Cazenave-Gassiot, A; Checa, A; Cinel, MA; Colas, RA; Cremers, S; Dennis, EA; Evans, JE; Fauland, A; Fiehn, O; Gardner, MS; Garrett, TJ; Gotlinger, KH; Han, J; Huang, Y; Neo, AH; Hyötyläinen, T; Izumi, Y; Jiang, H; Jiang, H; Jiang, J; Kachman, M; Kiyonami, R; Klavins, K; Klose, C; Köfeler, HC; Kolmert, J; Koal, T; Koster, G; Kuklenyik, Z; Kurland, IJ; Leadley, M; Lin, K; Maddipati, KR; McDougall, D; Meikle, PJ; Mellett, NA; Monnin, C; Moseley, MA; Nandakumar, R; Oresic, M; Patterson, R; Peake, D; Pierce, JS; Post, M; Postle, AD; Pugh, R; Qiu, Y; Quehenberger, O; Ramrup, P; Rees, J; Rembiesa, B; Reynaud, D; Roth, MR; Sales, S; Schuhmann, K; Schwartzman, ML; Serhan, CN; Shevchenko, A; Somerville, SE; St John-Williams, L; Surma, MA; Takeda, H; Thakare, R; Thompson, JW; Torta, F; Triebl, A; Trötzmüller, M; Ubhayasekera, SJK; Vuckovic, D; Weir, JM; Welti, R; Wenk, MR; Wheelock, CE; Yao, L; Yuan, M; Zhao, XH; Zhou, S

Published Date

  • December 2017

Published In

Volume / Issue

  • 58 / 12

Start / End Page

  • 2275 - 2288

PubMed ID

  • 28986437

Pubmed Central ID

  • PMC5711491

Electronic International Standard Serial Number (EISSN)

  • 1539-7262

Digital Object Identifier (DOI)

  • 10.1194/jlr.M079012


  • eng

Conference Location

  • United States