Comparison of the positional cloning methods used to isolate the BRCA1 gene.


Journal Article

A critical step in positional cloning is the identification of candidate genes from a large, genetically defined region. Candidate gene isolation by hybrid selection, genomic sequencing, and direct cDNA library screening identified 45 candidate gene fragments (CGFs) from a 600 kb genomic region that contains the BRCA1 gene. These CGFs define a minimum of 15 genes, six of which are newly localized to the BRCA1 region. We present an analysis of the efficiency and the sequences generated for each of these methods. We also compare our CGF set to those reported for the BRCA1 region by three other groups, revealing a surprising lack of overlap among the sets.

Full Text

Cited Authors

  • Harshman, K; Bell, R; Rosenthal, J; Katcher, H; Miki, Y; Swenson, J; Gholami, Z; Frye, C; Ding, W; Dayananth, P

Published Date

  • August 1995

Published In

Volume / Issue

  • 4 / 8

Start / End Page

  • 1259 - 1266

PubMed ID

  • 7581362

Pubmed Central ID

  • 7581362

Electronic International Standard Serial Number (EISSN)

  • 1460-2083

International Standard Serial Number (ISSN)

  • 0964-6906

Digital Object Identifier (DOI)

  • 10.1093/hmg/4.8.1259


  • eng