Isolation and properties of beta-glucosidase from Ruminococcus albus.

Journal Article (Journal Article)

An enzyme active against p-nitrophenyl-beta-D-glucoside was purified from logarithmic-phase cells of Ruminococcus albus cultivated in a medium containing ball-milled cellulose. The purification yielded homogeneous enzyme after an approximately 520-fold increase in specific activity and a 9% yield. The enzyme was identified as a beta-glucosidase because it can hydrolyze cellobiose and cellooligosaccharides to glucose from the nonreducing ends.

Full Text

Duke Authors

Cited Authors

  • Ohmiya, K; Shirai, M; Kurachi, Y; Shimizu, S

Published Date

  • January 1, 1985

Published In

Volume / Issue

  • 161 / 1

Start / End Page

  • 432 - 434

PubMed ID

  • 3918007

Pubmed Central ID

  • PMC214890

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.161.1.432-434.1985

Language

  • eng

Conference Location

  • United States