Isolation and properties of beta-glucosidase from Ruminococcus albus.
Journal Article (Journal Article)
An enzyme active against p-nitrophenyl-beta-D-glucoside was purified from logarithmic-phase cells of Ruminococcus albus cultivated in a medium containing ball-milled cellulose. The purification yielded homogeneous enzyme after an approximately 520-fold increase in specific activity and a 9% yield. The enzyme was identified as a beta-glucosidase because it can hydrolyze cellobiose and cellooligosaccharides to glucose from the nonreducing ends.
Full Text
Duke Authors
Cited Authors
- Ohmiya, K; Shirai, M; Kurachi, Y; Shimizu, S
Published Date
- January 1, 1985
Published In
Volume / Issue
- 161 / 1
Start / End Page
- 432 - 434
PubMed ID
- 3918007
Pubmed Central ID
- PMC214890
International Standard Serial Number (ISSN)
- 0021-9193
Digital Object Identifier (DOI)
- 10.1128/jb.161.1.432-434.1985
Language
- eng
Conference Location
- United States