A noncanonical function of cGAMP in inflammasome priming and activation.

Published

Journal Article

Recognition of pathogen-associated molecular patterns and danger-associated molecular patterns by host cells is an important step in innate immune activation. The DNA sensor cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) synthase (cGAS) binds to DNA and produces cGAMP, which in turn binds to stimulator of interferon genes (STING) to activate IFN-I. Here we show that cGAMP has a noncanonical function in inflammasome activation in human and mouse cells. Inflammasome activation requires two signals, both of which are activated by cGAMP. cGAMP alone enhances expression of inflammasome components through IFN-I, providing the priming signal. Additionally, when combined with a priming signal, cGAMP activates the inflammasome through an AIM2, NLRP3, ASC, and caspase-1 dependent process. These two cGAMP-mediated functions, priming and activation, have differential requirements for STING. Temporally, cGAMP induction of IFN-I precedes inflammasome activation, which then occurs when IFN-I is waning. In mice, cGAS/cGAMP amplify both inflammasome and IFN-I to control murine cytomegalovirus. Thus, cGAMP activates the inflammasome in addition to IFN-I, and activation of both is needed to control infection by a DNA virus.

Full Text

Duke Authors

Cited Authors

  • Swanson, KV; Junkins, RD; Kurkjian, CJ; Holley-Guthrie, E; Pendse, AA; El Morabiti, R; Petrucelli, A; Barber, GN; Benedict, CA; Ting, JP-Y

Published Date

  • December 4, 2017

Published In

Volume / Issue

  • 214 / 12

Start / End Page

  • 3611 - 3626

PubMed ID

  • 29030458

Pubmed Central ID

  • 29030458

Electronic International Standard Serial Number (EISSN)

  • 1540-9538

Digital Object Identifier (DOI)

  • 10.1084/jem.20171749

Language

  • eng

Conference Location

  • United States