Proacrosin/acrosin quantification as an indicator of acrosomal integrity in fresh and frozen dog spermatozoa.

Published

Journal Article

The scope of the present study was to evaluate the presence and activation of proacrosin/acrosin as a tool to determine the acrosomal status of fresh and frozen/thawed dog spermatozoa. Monoclonal antibody C5F11, directed against human acrosin, cross-reacted with dog spermatozoa and labeled the acrosome of both fresh and frozen/thawed dog spermatozoa. Frozen/thawed spermatozoa had a lesser proportion of labeled spermatozoa than fresh spermatozoa (P<0.05). When live spermatozoa were labeled with soybean trypsin inhibitor conjugated with Alexa 488 (SBTI-Alexa 488), the proportion of acrosome-labeled fresh spermatozoa was less than frozen/thawed spermatozoa (P<0.05). By using Western blots and enzymatic activity, frozen/thawed spermatozoa had a greater proportion of active acrosin than fresh spermatozoa. In addition, beta 1,4-galactosyl-transferase (GalT), a plasma membrane bound protein, remained attached to frozen/thawed spermatozoa. Proacrosin is activated during freezing/thawing of dog spermatozoa, and that proacrosin/acrosin may be a good indicator of acrosomal integrity of frozen/thawed spermatozoa.

Full Text

Duke Authors

Cited Authors

  • Cortes, CJ; Codelia, VA; Manosalva, I; de Lange, J; De Los Reyes, M; Moreno, RD

Published Date

  • June 2006

Published In

Volume / Issue

  • 93 / 1-2

Start / End Page

  • 165 - 175

PubMed ID

  • 16169165

Pubmed Central ID

  • 16169165

Electronic International Standard Serial Number (EISSN)

  • 1873-2232

International Standard Serial Number (ISSN)

  • 0378-4320

Digital Object Identifier (DOI)

  • 10.1016/j.anireprosci.2005.08.001

Language

  • eng