daf-16/FoxO promotes gluconeogenesis and trehalose synthesis during starvation to support survival.
Journal Article (Journal Article)
daf-16/FoxO is required to survive starvation in Caenorhabditis elegans, but how daf-16IFoxO promotes starvation resistance is unclear. We show that daf-16/FoxO restructures carbohydrate metabolism by driving carbon flux through the glyoxylate shunt and gluconeogenesis and into synthesis of trehalose, a disaccharide of glucose. Trehalose is a well-known stress protectant, capable of preserving membrane organization and protein structure during abiotic stress. Metabolomic, genetic, and pharmacological analyses confirm increased trehalose synthesis and further show that trehalose not only supports survival as a stress protectant but also serves as a glycolytic input. Furthermore, we provide evidence that metabolic cycling between trehalose and glucose is necessary for this dual function of trehalose. This work demonstrates that daf-16/FoxO promotes starvation resistance by shifting carbon metabolism to drive trehalose synthesis, which in turn supports survival by providing an energy source and acting as a stress protectant.
Full Text
Duke Authors
Cited Authors
- Hibshman, JD; Doan, AE; Moore, BT; Kaplan, RE; Hung, A; Webster, AK; Bhatt, DP; Chitrakar, R; Hirschey, MD; Baugh, LR
Published Date
- October 24, 2017
Published In
Volume / Issue
- 6 /
PubMed ID
- 29063832
Pubmed Central ID
- PMC5655125
Electronic International Standard Serial Number (EISSN)
- 2050-084X
Digital Object Identifier (DOI)
- 10.7554/eLife.30057
Language
- eng
Conference Location
- England