Phosphorylation of Aspergillus fumigatus PkaR impacts growth and cell wall integrity through novel mechanisms.

Published

Journal Article (Letter)

Protein kinase A (PKA) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography-tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subunit PkaR, distinct from those previously identified in mammals and yeasts, and demonstrated the importance of two phosphorylation clusters for hyphal growth and cell wall-stress response. We also identified key differences in the regulation of PKA subcellular localization in A. fumigatus compared with other species. This is the first analysis of the phosphoregulation of a PKA regulatory subunit in a filamentous fungus and uncovers critical mechanistic differences between PKA regulation in filamentous fungi compared with mammals and yeast species, suggesting divergent targeting opportunities.

Full Text

Duke Authors

Cited Authors

  • Shwab, EK; Juvvadi, PR; Waitt, G; Soderblom, EJ; Moseley, MA; Nicely, NI; Steinbach, WJ

Published Date

  • November 9, 2017

Published In

Volume / Issue

  • 591 / 22

Start / End Page

  • 3730 - 3744

PubMed ID

  • 29067690

Pubmed Central ID

  • 29067690

Electronic International Standard Serial Number (EISSN)

  • 1873-3468

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1002/1873-3468.12886

Language

  • eng