Controlled human malaria infection of Tanzanians by intradermal injection of aseptic, purified, cryopreserved Plasmodium falciparum sporozoites.

Published

Journal Article

Controlled human malaria infection (CHMI) by mosquito bite has been used to assess anti-malaria interventions in > 1,500 volunteers since development of methods for infecting mosquitoes by feeding on Plasmodium falciparum (Pf) gametocyte cultures. Such CHMIs have never been used in Africa. Aseptic, purified, cryopreserved Pf sporozoites, PfSPZ Challenge, were used to infect Dutch volunteers by intradermal injection. We conducted a double-blind, placebo-controlled trial to assess safety and infectivity of PfSPZ Challenge in adult male Tanzanians. Volunteers were injected intradermally with 10,000 (N = 12) or 25,000 (N = 12) PfSPZ or normal saline (N = 6). PfSPZ Challenge was well tolerated and safe. Eleven of 12 and 10 of 11 subjects, who received 10,000 and 25,000 PfSPZ respectively, developed parasitemia. In 10,000 versus 25,000 PfSPZ groups geometric mean days from injection to Pf positivity by thick blood film was 15.4 versus 13.5 (P = 0.023). Alpha-thalassemia heterozygosity had no apparent effect on infectivity. PfSPZ Challenge was safe, well tolerated, and infectious.

Full Text

Duke Authors

Cited Authors

  • Shekalaghe, S; Rutaihwa, M; Billingsley, PF; Chemba, M; Daubenberger, CA; James, ER; Mpina, M; Ali Juma, O; Schindler, T; Huber, E; Gunasekera, A; Manoj, A; Simon, B; Saverino, E; Church, LWP; Hermsen, CC; Sauerwein, RW; Plowe, C; Venkatesan, M; Sasi, P; Lweno, O; Mutani, P; Hamad, A; Mohammed, A; Urassa, A; Mzee, T; Padilla, D; Ruben, A; Sim, BKL; Tanner, M; Abdulla, S; Hoffman, SL

Published Date

  • September 2014

Published In

Volume / Issue

  • 91 / 3

Start / End Page

  • 471 - 480

PubMed ID

  • 25070995

Pubmed Central ID

  • 25070995

Electronic International Standard Serial Number (EISSN)

  • 1476-1645

Digital Object Identifier (DOI)

  • 10.4269/ajtmh.14-0119

Language

  • eng

Conference Location

  • United States