A mouse model of adult-onset anaemia due to erythropoietin deficiency.

Published

Journal Article

Erythropoietin regulates erythropoiesis in a hypoxia-inducible manner. Here we generate inherited super-anaemic mice (ISAM) as a mouse model of adult-onset anaemia caused by erythropoietin deficiency. ISAM express erythropoietin in the liver but lack erythropoietin production in the kidney. Around weaning age, when the major erythropoietin-producing organ switches from the liver to the kidney, ISAM develop anaemia due to erythropoietin deficiency, which is curable by administration of recombinant erythropoietin. In ISAM severe chronic anaemia enhances transgenic green fluorescent protein and Cre expression driven by the complete erythropoietin-gene regulatory regions, which facilitates efficient labelling of renal erythropoietin-producing cells. We show that the majority of cortical and outer medullary fibroblasts have the innate potential to produce erythropoietin, and also reveal a new set of erythropoietin target genes. ISAM are a useful tool for the evaluation of erythropoiesis-stimulating agents and to trace the dynamics of erythropoietin-producing cells.

Full Text

Duke Authors

Cited Authors

  • Yamazaki, S; Souma, T; Hirano, I; Pan, X; Minegishi, N; Suzuki, N; Yamamoto, M

Published Date

  • 2013

Published In

Volume / Issue

  • 4 /

Start / End Page

  • 1950 -

PubMed ID

  • 23727690

Pubmed Central ID

  • 23727690

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/ncomms2950

Language

  • eng

Conference Location

  • England