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Methylglyoxal augments intracellular oxidative stress in human aortic endothelial cells.

Publication ,  Journal Article
Miyazawa, N; Abe, M; Souma, T; Tanemoto, M; Abe, T; Nakayama, M; Ito, S
Published in: Free Radic Res
January 2010

Methylglyoxal (MGO) is a non-enzymatic metabolite in the glycolytic pathway and its concentration in blood and tissues is elevated in diabetes and renal failure. MGO induces tissue injuries via ROS; however, the mechanism remains to be clarified. The present study examined the harmful actions of MGO. Human aortic endothelial cells were assessed under real-time fluorescent microscopy with continuous superfusion. Increases in intracellular ROS were measured with fluorescent indicator, 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate acetyl ester (DCFH-DA). The addition of MGO rapidly increased the ROS in a dose-dependent manner. The increment of DCF was entirely abolished by pre-treatment with superoxide anion scavenger and membrane-permeable catalase, indicating that MGO induces superoxide production. The increment was completely inhibited by 2-thenoyltrifluoroacetone or carbonyl cyanide 3-chlorophenylhydrazone and partially inhibited by N-methyl-L-arginine. These data suggest that MGO stimulates superoxide production from mitochondria and partially stimulates nitric oxide synthase in human endothelial cells.

Duke Scholars

Published In

Free Radic Res

DOI

EISSN

1029-2470

Publication Date

January 2010

Volume

44

Issue

1

Start / End Page

101 / 107

Location

England

Related Subject Headings

  • Up-Regulation
  • Reactive Oxygen Species
  • Pyruvaldehyde
  • Oxidative Stress
  • Oxidants
  • Nitric Oxide Synthase
  • Intracellular Space
  • Humans
  • Fluoresceins
  • Endothelial Cells
 

Citation

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MLA
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Miyazawa, N., Abe, M., Souma, T., Tanemoto, M., Abe, T., Nakayama, M., & Ito, S. (2010). Methylglyoxal augments intracellular oxidative stress in human aortic endothelial cells. Free Radic Res, 44(1), 101–107. https://doi.org/10.3109/10715760903321788
Miyazawa, Noriko, Michiaki Abe, Tomokazu Souma, Masayuki Tanemoto, Takaaki Abe, Masaaki Nakayama, and Sadayoshi Ito. “Methylglyoxal augments intracellular oxidative stress in human aortic endothelial cells.Free Radic Res 44, no. 1 (January 2010): 101–7. https://doi.org/10.3109/10715760903321788.
Miyazawa N, Abe M, Souma T, Tanemoto M, Abe T, Nakayama M, et al. Methylglyoxal augments intracellular oxidative stress in human aortic endothelial cells. Free Radic Res. 2010 Jan;44(1):101–7.
Miyazawa, Noriko, et al. “Methylglyoxal augments intracellular oxidative stress in human aortic endothelial cells.Free Radic Res, vol. 44, no. 1, Jan. 2010, pp. 101–07. Pubmed, doi:10.3109/10715760903321788.
Miyazawa N, Abe M, Souma T, Tanemoto M, Abe T, Nakayama M, Ito S. Methylglyoxal augments intracellular oxidative stress in human aortic endothelial cells. Free Radic Res. 2010 Jan;44(1):101–107.

Published In

Free Radic Res

DOI

EISSN

1029-2470

Publication Date

January 2010

Volume

44

Issue

1

Start / End Page

101 / 107

Location

England

Related Subject Headings

  • Up-Regulation
  • Reactive Oxygen Species
  • Pyruvaldehyde
  • Oxidative Stress
  • Oxidants
  • Nitric Oxide Synthase
  • Intracellular Space
  • Humans
  • Fluoresceins
  • Endothelial Cells